Kinetics of low density lipoprotein interactions with rabbit aortic wall following balloon catheter deendothelialization

Journal Article · · Arteriosclerosis (Dallas); (United States)

DOI:https://doi.org/10.1161/01.ATV.4.4.395· OSTI ID:6551994

Alavi, M; Moore, S

The kinetics of the interaction of circulating lipoproteins with the balloon catheter-injured aortic wall of rabbits were investigated. The fate of radioiodinated low density lipoprotein (LDL) was followed up to 48 hours after intravenous injection and compared with radioiodinated albumin injected simultaneously. The accumulation of LDL was calculated from the radioactivity in the aortic tissue divided by the average specific activity, measured from the area below the plasma radioactivity versus the time curve, and expressed as cpm/ml/hr. It was observed that the aortic area of endothelial regeneration (AER), which is relatively impermeable to Evans blue dye and shows very little albumin concentration, accumulated considerably more LDL than control (uninjured) tissues. Furthermore, removal of labeled lipoprotein from the intima during a 24-hour efflux period following the initial 48-hour loading period indicated that there was greater removal from normal and deendothelialized aortic (DEA) tissues than from neointima covered by endothelium. These findings are consistent with previous observations indicating changes in the morphological and chemical composition of AER. There is evidence that greater neointimal lipid accumulation in response to endothelial injury parallels the concentration of glycosaminoglycans (GAG). The relatively increased accumulation of label in AER, while plasma-specific activity is rapidly decreasing, indicates that there may be an ionic interaction of GAG with LDL in the AER.

• Research Organization: Department of Pathology, McMaster University Medical Centre, Hamilton, Ontario, Canada
• OSTI ID: 6551994
• Journal Information: Arteriosclerosis (Dallas); (United States), Journal Name: Arteriosclerosis (Dallas); (United States) Vol. 4:4; ISSN ARTRD
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALBUMINS
ANIMALS
AORTA
ARTERIES
BIOCHEMICAL REACTION KINETICS
BLOOD VESSELS
BODY
CARDIOVASCULAR SYSTEM
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIPIDS
LIPOPROTEINS
MAMMALS
MATERIALS
MEDICAL SUPPLIES
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RABBITS
REACTION KINETICS
SURGICAL MATERIALS
TRACER TECHNIQUES
VERTEBRATES