Accumulation of native and methylated low density lipoproteins by healing rabbit arterial wall

Journal Article · Wed Jul 01 04:00:00 EDT 1987 · Arteriosclerosis (Dallas); (United States)

DOI:https://doi.org/10.1161/01.ATV.7.4.361· OSTI ID:6075566

Fischman, A J; Lees, A M; Lees, R S; Barlai-Kovach, M; Strauss, H W

To determine whether healing arterial wall accumulation of low density lipoproteins (LDL) is mediated by the high affinity LDL receptor, normocholesterolemic rabbits were injected with /sup 125/I-LDL, /sup 99m/Tc-LDL, or the reductively methylated analogs of these compounds (/sup 125/I-MeLDL, /sup 99m/Tc-MeLDL), 1 month after balloon catheter deendothelialization of the abdominal aorta. If the mechanism of accumulation requires interaction with the LDL receptor, reductively methylated lipoproteins which do not bind to the receptor should not accumulate in healing arterial wall. Twenty-four hours after injection of labelled lipoproteins, each animal was injected with Evans blue dye, in order to distinguish reendothelialized from deendothelialized aorta. One hour after dye injection, the aorta was fixed, removed, divided into abdominal (ballooned) and thoracic (unballooned) regions and counted. For all lipoprotein preparations, there were three to four times as many counts in the abdominal as in the thoracic aorta. En face autoradiographs were made of the aortas that had been exposed to /sup 125/I-labelled lipoproteins. In the autoradiographs, the areas of the lowest activity corresponded to the centers of healing endothelial islands. The most intense radioactivity for both lipoproteins occurred in the region of the leading edge of the endothelial islands where active endothelial regeneration was in progress. The overall distribution of native and MeLDL accumulation was the same. The results suggest that low density lipoproteins are accumulated in areas of active endothelial regeneration by a mechanism that does not involve the high affinity LDL receptor.

• Research Organization: Massachusetts General Hospital, Boston
• OSTI ID: 6075566
• Journal Information: Arteriosclerosis (Dallas); (United States), Journal Name: Arteriosclerosis (Dallas); (United States) Vol. 7:4; ISSN ARTRD
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ADRENAL GLANDS
ANIMAL TISSUES
ANIMALS
AORTA
ARTERIES
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL ACCUMULATION
BIOLOGICAL RECOVERY
BIOLOGICAL REGENERATION
BLOOD VESSELS
BODY
CARDIOVASCULAR SYSTEM
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ENDOCRINE GLANDS
ENDOTHELIUM
GLANDS
HEALING
INJURIES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOMERIC NUCLEI
ISOTOPES
LIPIDS
LIPOPROTEINS
MAMMALS
MEMBRANE PROTEINS
METHYLATION
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RABBITS
RADIOISOTOPES
RECEPTORS
RECOVERY
TECHNETIUM 109
TECHNETIUM ISOTOPES
TISSUES
VERTEBRATES
WOUNDS