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Effects of X rays on BCNU-induced DNA crosslinking

Journal Article · · Radiat. Res.; (United States)
DOI:https://doi.org/10.2307/3576454· OSTI ID:6864865
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The technique of alkaline elution has been used to study DNA interstrand crosslinking in 9L rat brain tumor cells treated with combinations of 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) and X rays. Irradiation with doses as low as 50 rad of X rays immediately or 6 hr after a 1-hr treatment with 60, 80, or 100 ..mu..M BCNU produced a significant increase in BCNU-induced DNA interstrand crosslinking. If cells were irradiated before BCNU treatment, the amount of crosslinking was not affected compared with BCNU alone. As found in the crosslinking studies, irradiation immediately or 6 hr after the BCNU treatment produced enhanced cell kill, but irradiation 6 hr before BCNU treatment did not produce enhanced cell kill. Therefore, the X-ray-mediated increase in BCNU-induced DNA interstrand crosslinking may be the mechanism through which cell kill is increased by combination treatment with the agents.
Research Organization:
Univ. of California, San Francisco
OSTI ID:
6864865
Journal Information:
Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 99:1; ISSN RAREA
Country of Publication:
United States
Language:
English

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Related Subjects

560111 -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BODY
BRAIN
CELL KILLING
CENTRAL NERVOUS SYSTEM
CHEMICAL REACTIONS
CROSS-LINKING
DATA
DNA
ELECTROMAGNETIC RADIATION
EXPERIMENTAL DATA
INFORMATION
IONIZING RADIATIONS
MAMMALS
NERVOUS SYSTEM
NITROSO COMPOUNDS
NITROSOUREAS
NUCLEIC ACIDS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
POLYMERIZATION
RADIATIONS
RADIOINDUCTION
RADIOSENSITIVITY EFFECTS
RATS
RODENTS
SURVIVAL CURVES
TUMOR CELLS
VERTEBRATES
X RADIATION