BCNU-induced sister chromatid exchanges are increased by X irradiation
Journal Article
·
· Radiat. Res.; (United States)
The authors have studied the effect on sister chromatid exchange (SCE) induction in 9L rat brain tumor cells caused by combination treatment with 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) and X rays. Over the dose and concentration ranges used in these experiments, BCNU induced relatively large numbers of SCEs, while X rays induced few SCEs. When cells were X irradiated immediately after BCNU treatment, the number of SCEs induced was greater that the number of SCEs expected by adding the number of SCEs induced by each agent alone; the number of SCEs induced as a result of this BCNU-X-ray interaction increased as the concentration of BCNU and/or dose of X rays increased. When the addition of bromodeoxyuridine was delayed from 0 to 16 hr after BCNU treatment, the number of SCEs induced declined to control levels by 16 hr. If X irradiation was delayed for up to 16 hr after BCNU treatment the same pattern of decrease was observed; the number of SCEs induced at each time point, however, was greater than that induced by BCNU and X rays alone. X irradiation from 0-16 hr before BCNU treatment produced the same number of SCEs as that produced by BCNU alone. Thus the SCE assay is capable of detecting a drug-X-ray interaction in mammalian cells and provides a sensitive means of studying the sequencing and timing that leads to the interaction.
- Research Organization:
- Univ. of California, San Francisco
- OSTI ID:
- 5245565
- Journal Information:
- Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 97:1; ISSN RAREA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
CHROMOSOMAL ABERRATIONS
ELECTROMAGNETIC RADIATION
IONIZING RADIATIONS
MAMMALS
MUTATIONS
NERVOUS SYSTEM
NITROSO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
RADIATIONS
RADIOINDUCTION
RADIOSENSITIVITY
RADIOSENSITIVITY EFFECTS
RATS
RODENTS
SISTER CHROMATID EXCHANGES
SYNERGISM
TUMOR CELLS
VERTEBRATES
X RADIATION
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
CHROMOSOMAL ABERRATIONS
ELECTROMAGNETIC RADIATION
IONIZING RADIATIONS
MAMMALS
MUTATIONS
NERVOUS SYSTEM
NITROSO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
RADIATIONS
RADIOINDUCTION
RADIOSENSITIVITY
RADIOSENSITIVITY EFFECTS
RATS
RODENTS
SISTER CHROMATID EXCHANGES
SYNERGISM
TUMOR CELLS
VERTEBRATES
X RADIATION