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Comparison of 6-thioguanine-resistant mutation and sister chromatid exchanges in Chinese hamster V79 cells with forty chemical and physical agents

Journal Article · · Cancer Res.; (United States)
OSTI ID:6304795
; ; ; ;
The induction of sister chromatid exchanges (SCE) and mutation at the hypoxanthine-guanine phosphoribosyl transferase locus and toxicities of 40 different chemical and physical agents were examined on Chinese hamster V79 cells. These agents included mono-, di-, tri-, and polyfunctional alkylating agents, intercalators, gamma-rays, and UV light irradiation. Mutation was measured as resistance to 6-thioguanine and toxicity as loss of cell-plating efficiency. SCE were examined 29 hr after treatment. With the agents examined, a highly positive correlation existed between SCE-inducing and mutagenic potencies, when expressed as increase in the number per a unit dose over the control values. But the great difference of the ratios of mutagenic potencies versus SCE-inducing potencies among agents was observed, the maximal difference in the ratios being about 200-fold. The agents that showed the higher values of the ratio (agents producing more mutations than SCE) were bleomycin, cobalt-60 gamma-rays, all ethylating agents (N-ethyl-N-nitrosourea, N-ethyl-N'-nitro-N-nitrosoguanidine, ethyl methanesulfonate, and diethylsulfate), N-propyl-N-nitrosourea, N-butyl-N-nitrosourea, isopropyl methanesulfonate, intercalating acridine compounds (2-methoxy-6-chloro-9-(3-(ethyl-2-chloroethyl)aminopropylamino)-acridine X 2HCl and 2-methoxy-6-chloro-9-(3-(chloroethyl)-aminopropylamino)acridine 2HCl) and UV light at 254 nm.
Research Organization:
Japan Tobacco and Salt Public Corp., Kanagawa
OSTI ID:
6304795
Journal Information:
Cancer Res.; (United States), Journal Name: Cancer Res.; (United States) Vol. 44:8; ISSN CNREA
Country of Publication:
United States
Language:
English

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Related Subjects

560121 -- Radiation Effects on Cells-- External Source-- (-1987)
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACRIDINES
ALKYLATING AGENTS
ANIMAL CELLS
AROMATICS
AZAARENES
AZINES
CHO CELLS
CHROMOSOMAL ABERRATIONS
ELECTROMAGNETIC RADIATION
GAMMA RADIATION
HETEROCYCLIC COMPOUNDS
IONIZING RADIATIONS
MUTAGENESIS
MUTATIONS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIDINES
RADIATIONS
SISTER CHROMATID EXCHANGES
TOXICITY
ULTRAVIOLET RADIATION