Differential responses to radiation and hyperthermia of cloned cell lines derived from a single human melanoma xenograft

Journal Article · Fri Jun 01 04:00:00 EDT 1984 · Int. J. Radiat. Oncol., Biol. Phys.; (United States)

DOI:https://doi.org/10.1016/0360-3016(84)90388-2· OSTI ID:6864862

Rofstad, E K; Brustad, T

One uncloned and five cloned cell lines were derived from a single human melanoma xenograft. Cells from passages 7-12 were exposed to either radiation or hyperthermia (42.5/sup 0/C, pH = 7.4) under aerobic conditions and the colony forming ability of the cells was assayed in soft agar. The five cloned lines showed individual and characteristic responses to radiation as well as to hyperthermia. The variation in the response to radiation was mainly reflected in the size of the shoulders of the survival curves rather than in the D/sub 0/-values. The variation in the response to hyperthermia was mainly reflected in the terminal slopes of the survival curves. The survival curve of cells from the uncloned line, both when exposed to radiation and hyperthermia, was positioned in the midst of those of the cloned lines. The response of the cloned lines to radiation did not correlate with the response to hyperthermia, indicating that tumor cell subpopulations which are resistant to radiation may respond well to hyperthermia.

• Research Organization: The Norwegian Radium Hospital, Oslo
• OSTI ID: 6864862
• Journal Information: Int. J. Radiat. Oncol., Biol. Phys.; (United States), Journal Name: Int. J. Radiat. Oncol., Biol. Phys.; (United States) Vol. 10:6; ISSN IOBPD
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY TEMPERATURE
CELL CULTURES
CELL FLOW SYSTEMS
CELL KILLING
CLONE CELLS
DISEASES
ELECTROMAGNETIC RADIATION
HYPERTHERMIA
IN VITRO
IONIZING RADIATIONS
MAMMALS
MAN
MELANOMAS
NEOPLASMS
PRIMATES
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RADIOSENSITIVITY EFFECTS
SURVIVAL CURVES
TUMOR CELLS
VERTEBRATES
X RADIATION