Spontaneous mitotic recombination in the mms8-1 mutant of Saccharomyces cerevisiae

Technical Report · Mon Jan 01 04:00:00 EST 1979

OSTI ID:6864817

Montelone, B A; Prakash, S; Prakash, L

The MMS sensitive mutation mms8-1 increases the rate of spontaneous mitotic intragenic recombination at five heteroallelic loci on three chromosomes. Complementation, segregation, and mapping studies indicate that mms8-1 is allelic to cdc9, known to be defective in DNA ligase. Both mms8-1 and cdc9 mutants are lethal in combination with the recombination defective mutant rad52-1. Genetic analysis of spontaneous red/white sectors in an ade2-1/ade2-1 ade5/+ mms8-1/mms8-1 strain shows non-reciprocal recombinational events involving long chromosome segments. We also observe increased rates of simultaneous recombination at loci on different chromosomes in both wild type and mms8-1 mutants.

• Research Organization: Rochester Univ., NY (USA)
• OSTI ID: 6864817
• Report Number(s): UR-3490-1980
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
CELL DIVISION
DATA
ESTERS
EXPERIMENTAL DATA
FUNGI
GENE RECOMBINATION
INFORMATION
METHYL METHANESULFONATE
MICROORGANISMS
MITOSIS
MUTAGENS
MUTANTS
MUTATIONS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PLANTS
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
SPONTANEOUS MUTATIONS
SULFONIC ACID ESTERS
YEASTS