Spontaneous mitotic recombination in mms8-1, an allele of the CDC9 gene of Saccharomyces cerevisiae

Journal Article · Sat Aug 01 04:00:00 EDT 1981 · J. Bacteriol.; (United States)

OSTI ID:6230126

Montelone, B A; Prakash, S; Prakash, L

The methyl methane sulfonate (MMS)-sensitive mutation mms8-1 increases the rate of spontaneous mitotic intragenic recombination at five heteroallelic loci on three chromosomes. Complementation, segregation, and mapping studies indicate that mms8-1 is allelic to cdc9, known to be defective in deoxyribonucleic acid ligase. Both mms8-1 and cdc9 mutants are lethal in combination with the recombination-defective mutant rad52-1. Genetic analysis of spontaneous red/white sectors in an ade2-1/ade2-1 ade5/ + mms8-1/mms8-1 strain shows nonreciprocal recombinational events involving long chromosome segments. We also observe greater than expected rates of simultaneous recombination at loci on different chromosomes in both wild-type and mms8-1 mutants.

• Research Organization: Univ. of Rochester, NY
• DOE Contract Number: AC02-76EV03490
• OSTI ID: 6230126
• Journal Information: J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 147:2; ISSN JOBAA
• Country of Publication: United States
• Language: English

Related Subjects

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550400 -- Genetics
550700* -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
BIOLOGICAL EFFECTS
CELL DIVISION
CHROMOSOMES
DNA
ENZYME ACTIVITY
ENZYMES
ESTERS
FUNGI
GENE RECOMBINATION
GENES
GENETIC EFFECTS
GENETIC MAPPING
LIGASES
MAPPING
METABOLISM
METHYL METHANESULFONATE
MICROORGANISMS
MITOSIS
MUTAGENESIS
MUTAGENS
MUTANTS
MUTATIONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PLANTS
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
SPONTANEOUS MUTATIONS
SULFONIC ACID ESTERS
YEASTS