Quantitative autoradiographic measurement of regional myocardial substrate uptake in hypertensive rats

Conference · Sat Dec 31 23:00:00 EST 1983 · J. Nucl. Med.; (United States)

OSTI ID:6860554

Yonekura, Y; Tamaki, N; Torizuka, K; Brill, A B; Som, P; Yamamoto, K; Srivastava, S; Iwai, J; Elmaleh, D R; Livni, E

Quantitative double tracer autoradiographic technique permits to study the distribution of two tracers in the same section of small animals with high spatial resolution. The authors have applied this technique to measure regional myocardial perfusion and substrate uptake in hypertensive rats. To compate regional myocardial uptake of fatty acid and glucose analogs with regional myocardial perfusion, three different double tracer experiments were conducted utilizing the difference of physical half-lives as follows: I) (C-14)3-methyl-heptadecanoic acid (BMHDA) and T1-201 (TL), II) (C-14)2-deoxy-D-glucose (DG) and TL, III) (F-18)2-fluoro-2-deoxy-D-glucose (FDG) and BMHDA. DG (or FDG), BMHDA and TL were administered to rats 45 min, 15 min and 5 min prior to sacrifice, respectively. The autoradiograms were digitized and quantitated using a videodensitometry film analysis system. Myocardial perfusion as indicated by TL distribution was homogeneous in both normotensive and hypertensive rats. However, hypertensive myocardium showed decreased uptake of BMHDA in the endocardial and septal regions, where DG showed markedly increased uptake. Double tracer experiment with BMHDA and FDG clearly demonstrated this complementary relation in the same section. These data indicate that hypertensive myocardium was abnormal substrate utilization although regional myocardial perfusion is not impaired. Moreover, (C-11)BMHDA and (F-18)FDG may be used to measure regional myocardial substrate utilization in humans with positron emission tomography.

• Research Organization: Kyoto Univ., Kyoto
• OSTI ID: 6860554
• Report Number(s): CONF-840619-
• Journal Information: J. Nucl. Med.; (United States), Journal Name: J. Nucl. Med.; (United States) Vol. 25:5; ISSN JNMEA
• Country of Publication: United States
• Language: English

Related Subjects

550602* -- Medicine-- External Radiation in Diagnostics-- (1980-)
62 RADIOLOGY AND NUCLEAR MEDICINE
ALDEHYDES
ANIMALS
AUTORADIOGRAPHY
BODY
CARBOHYDRATES
CARBOXYLIC ACIDS
CARDIOVASCULAR DISEASES
CARDIOVASCULAR SYSTEM
COMPUTERIZED TOMOGRAPHY
COUNTING TECHNIQUES
DIAGNOSTIC TECHNIQUES
DISEASES
DISTRIBUTION
DRUGS
DUAL-ISOTOPE SUBTRACTION TECHNIQUE
EMISSION COMPUTED TOMOGRAPHY
FUNCTIONS
GLUCOSE
HEART
HEXOSES
HYPERTENSION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MONOSACCHARIDES
MUSCLES
MYOCARDIUM
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
POSITRON COMPUTED TOMOGRAPHY
RADIOISOTOPE SCANNING
RADIONUCLIDE KINETICS
RADIOPHARMACEUTICALS
RATS
RETENTION FUNCTIONS
RODENTS
SACCHARIDES
SCINTISCANNING
SYMPTOMS
TISSUE DISTRIBUTION
TOMOGRAPHY
TRACER TECHNIQUES
UPTAKE
VASCULAR DISEASES
VERTEBRATES