Membrane-associated precursor to poliovirus VPg identified by immunoprecipitation with antibodies directed against a synthetic heptapeptide

Journal Article · Mon Feb 01 04:00:00 EST 1982 · Cell; (United States)

DOI:https://doi.org/10.1016/0092-8674(82)90358-0· OSTI ID:6857527

Semelr, B L ^[1]; Anderson, C W; Hanecak, R; Dorner, L F; Wimmer, E

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1. State Univ. of New York, Stony Brook

A synthetic heptapeptide corresponding to the C-terminal sequence of the poliovirus genome protein (VPg) has been linked to bovine serum albumin and used to raise antibodies in rabbits. These antibodies precipitate not only VPg but also at least two more virus-specific polypeptides. The smaller polypeptide, denoted P3-9 (12,000 daltons), has been mapped by Edman degradation and by fragmentation with cyanogen bromide and determined to be the N-terminal cleavage product of polypeptide P3-1b, a precursor to the RNA polymerase. P3-9 contains the sequence of the basic protein VPg (22 amino acids) at its C terminus. As predicted by the known RNA sequence of poliovirus, P3-9 also contains a hydrophobic region of 22 amino acids preceding VPg, an observation suggesting that P3-9 may be membrane-associated. This was confirmed by fractionation of infected cells in the presence or absence of detergent. We speculate that P3-9 may be the donor of VPg to RNA chains in the membrane-bound RNA replication complex.

• OSTI ID: 6857527
• Journal Information: Cell; (United States), Journal Name: Cell; (United States) Vol. 28; ISSN CELLB
• Country of Publication: United States
• Language: English

Related Subjects

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59 BASIC BIOLOGICAL SCIENCES
ALBUMINS
AMINO ACIDS
ANIMALS
ANTIBODY FORMATION
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BIOCHEMISTRY
BROMIDES
BROMINE COMPOUNDS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL MEMBRANES
CHEMISTRY
CYANOGEN
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ELECTROPHORESIS
HALIDES
HALOGEN COMPOUNDS
HELA CELLS
HYDROXY ACIDS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
MAMMALS
MEMBRANES
MICROORGANISMS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PEPTIDES
POLIO VIRUS
POLYPEPTIDES
PRECIPITATION
PROTEINS
RABBITS
RADIOISOTOPES
SEPARATION PROCESSES
TRITIUM COMPOUNDS
TYROSINE
VERTEBRATES
VIRUSES