Poliovirus RNA synthesis in vitro: structural elements and antibody inhibition
Journal Article
·
· Virology; (United States)
The poliovirus RNA polymerase complex has been analyzed by immunoautoradiography using antibody probes derived from purified replicase (P3) region viral polypeptides. Antibody preparations made against the polio RNA polymerase, P3-4b, detected a previously unreported cellular protein that copurifies with the RNA polymerase. An IgG fraction purified from rabbit antiserum to polypeptide P3-2, a precursor fo the RNA polymerase, specifically inhibits poliovirus RNA synthesis in vitro. The authors have also immunoprecipitated a 60,000-dalton protein (P3-4a) with antiserum to protein P3-4b and have determined the precise genomic map position of this protein by automated Edman degradation. Protein P3-4a originates by cleavage of the RNA polymerase precursor at a glutamine-glucine amino acid pair not previously reported to be a viral cleavage site.
- Research Organization:
- State Univ. of New York, Stony Brook
- OSTI ID:
- 6917412
- Journal Information:
- Virology; (United States), Journal Name: Virology; (United States) Vol. 126; ISSN VIRLA
- Country of Publication:
- United States
- Language:
- English
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Sun Jan 31 23:00:00 EST 1982
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OSTI ID:6857527
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· Proc. Natl. Acad. Sci. U.S.A.; (United States)
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Thesis/Dissertation
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Mon Dec 31 23:00:00 EST 1984
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OSTI ID:6941553
Related Subjects
550201* -- Biochemistry-- Tracer Techniques
550701 -- Microbiology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOSYNTHESIS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
EVEN-ODD NUCLEI
HYDROGEN ISOTOPES
IN VITRO
INHIBITION
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MAMMALS
MICROORGANISMS
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PARASITES
PHOSPHORUS-GROUP TRANSFERASES
POLIO VIRUS
POLYMERASES
PROTEINS
RABBITS
RADIOISOTOPES
RNA
SULFUR 35
SULFUR ISOTOPES
SYNTHESIS
TRACER TECHNIQUES
TRANSFERASES
TRITIUM
VERTEBRATES
VIRUSES
YEARS LIVING RADIOISOTOPES
550701 -- Microbiology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOSYNTHESIS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
EVEN-ODD NUCLEI
HYDROGEN ISOTOPES
IN VITRO
INHIBITION
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MAMMALS
MICROORGANISMS
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PARASITES
PHOSPHORUS-GROUP TRANSFERASES
POLIO VIRUS
POLYMERASES
PROTEINS
RABBITS
RADIOISOTOPES
RNA
SULFUR 35
SULFUR ISOTOPES
SYNTHESIS
TRACER TECHNIQUES
TRANSFERASES
TRITIUM
VERTEBRATES
VIRUSES
YEARS LIVING RADIOISOTOPES