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Poliovirus replication proteins: RNA sequence encoding P3-1b and the sites of proteolytic processing

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
 [1]; ; ; ; ;
  1. State Univ. of New York, Stony Brook
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A partial amino-terminal amino acid sequence of each of the major proteins encoded by the replicase region of the poliovirus genome has been determined. A comparison of this sequence information with the amino acid sequence predicted from the RNA sequence that has been determined for the 3' region of the poliovirus genome has allowed us to locate precisely the proteolytic cleavage sites at which the initial polyprotein is processed to create the poliovirus products P3-1b (NCVP1b), P3-2 (NCVP2), P3-4b (NCVP4b), and P3-7c (NCVP7c). For each of these products, as well as for the small genome-linked protein VPg, proteolytic cleavage occurs between a glutamine and a glycine residue to create the amino terminus of each protein. This result suggests that a single proteinase may be responsible for all of these cleavages. The sequence data also allow the precise positioning of the genome-linked protein VPg within the precursor P3-1b just proximal to the amino terminus of polypeptide P3-2.
OSTI ID:
6455460
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 78:6; ISSN PNASA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
550401 -- Genetics-- Tracer Techniques
550701 -- Microbiology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMIDES
AMINO ACIDS
BIOCHEMISTRY
BIOSYNTHESIS
CARBOXYLIC ACIDS
CELL CULTURES
CHEMICAL REACTIONS
CHEMISTRY
CLEAVAGE
CRYSTAL STRUCTURE
DECOMPOSITION
ENZYMES
GENETIC MAPPING
GLUTAMINE
GLYCINE
MAPPING
MICROORGANISMS
MICROSTRUCTURE
NUCLEIC ACIDS
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARASITES
POLIO VIRUS
PROTEINS
PROTEOLYSIS
RNA
STRUCTURAL CHEMICAL ANALYSIS
SYNTHESIS
VIRUSES