Cleavage sites in the polypeptide precursors of poliovirus protein P2-X

Journal Article · Thu Jan 01 04:00:00 EST 1981 · Virology; (United States)

OSTI ID:5443440

Selmer, B L ^[1]; Hanecak, R; Anderson, C W; Wimmer, E

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1. State Univ. of New York, Stony Brook

Partial amino-terminal sequence analysis has been performed on the three major polypeptide products (P2-3b, P2-5b, and P2-X) from the central region (P2) of the poliovirus polyprotein, and this analysis precisely locates the amino termini of these products with respect to the nucleotide sequence of the poliovirus RNA genome. Like most of the products of the replicase region (P3), the amino termini of P2-5b and P2-X are generated by cleavage between glutamine and glycine residues. Thus, P2-5b and P2-X are probably both produced by the action of a singly (virus-encoded.) proteinase. The amino terminus of P2-3b, on the other hand, is produced by a cleavage between the carboxy-terminal tyrosine of VP1 and the glycine encoded by nucleotides 3381-3383. This result may suggest that more than one proteolytic activity is required for the complete processing of the poliovirus polyprotein.

• OSTI ID: 5443440
• Journal Information: Virology; (United States), Journal Name: Virology; (United States) Vol. 114; ISSN VIRLA
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400103 -- Radiometric & Radiochemical Procedures-- (-1987)
550201* -- Biochemistry-- Tracer Techniques
550701 -- Microbiology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMIDES
AMINO ACIDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
BIOSYNTHESIS
CARBOXYLIC ACIDS
CHEMICAL ANALYSIS
CHEMICAL REACTIONS
CHEMISTRY
DAYS LIVING RADIOISOTOPES
DECOMPOSITION
ENZYMES
EVEN-ODD NUCLEI
GLUTAMINE
GLYCINE
HYDROLASES
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MICROORGANISMS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARASITES
PEPTIDE HYDROLASES
PEPTIDES
POLIO VIRUS
POLYPEPTIDES
PROTEINS
PROTEOLYSIS
QUANTITATIVE CHEMICAL ANALYSIS
RADIOCHEMICAL ANALYSIS
RADIOISOTOPES
SULFUR 35
SULFUR ISOTOPES
SYNTHESIS
TRITIUM COMPOUNDS
VIRUSES