Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Decrease in hematopoietic stem cell domains as a delayed effect of x-irradiation

Journal Article · · Int. J. Cell Cloning; (United States)
; ;
Although the hematopoietic integrity of locally X-irradiated sites can be restored for a time even after fairly large doses, a secondary aplasia often occurs some months later. To gain further insight into this delayed effect within the framework of the stem cell regulatory domain hypothesis, we characterized the growth kinetics of spleen colony forming units (CFU-S) in WBB6FI-+/+ bone marrow transplanted into WBB6FI-W/WV mice in which one leg had been exposed to 10-30 Gy of X rays 4-5 months previously. Compared to unirradiated contralateral marrow, fewer CFU-S either reached the previously irradiated marrow or were seeded into sites that could support growth. The initial exponential growth of effectively seeded CFU-S was unchanged, but growth deceleration (inflection point) occurred at a lower level of CFU-S in marrow previously irradiated with 20-30 Gy. This change in the inflection point indicates a radiation dose-dependent decrease consistent with the decrease in bone marrow cellularity. The decrease in effective stem cell domains after 20 Gy was calculated to be about 35%. We interpret these results to reflect the highly localized nature of delayed radiation damage to the marrow microenvironment.
Research Organization:
Laboratory of Radiobiology and Environmental Health, University of California, San Francisco
OSTI ID:
6849145
Journal Information:
Int. J. Cell Cloning; (United States), Journal Name: Int. J. Cell Cloning; (United States) Vol. 1:4; ISSN IJCCE
Country of Publication:
United States
Language:
English

Similar Records

Observations on the contributions of environmental restraints and innate stem cell ability to hematopoietic regeneration
Journal Article · Mon Feb 29 23:00:00 EST 1988 · Int. J. Cell Cloning; (United States) · OSTI ID:6971604
Effects of low-level radiation upon the hematopoietic steam cell: implications for leukemogenesis
Technical Report · Fri Dec 31 23:00:00 EST 1982 · OSTI ID:6300559
The effects of x-irradiation on hematopoietic stem cell compartments in the mouse
Journal Article · Thu Aug 01 00:00:00 EDT 1991 · Experimental Hematology (Lawrence, Kansas); (United States) · OSTI ID:5018796

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BLOOD FORMATION
BODY
BONE MARROW
CFU
DELAYED RADIATION EFFECTS
ELECTROMAGNETIC RADIATION
HEMATOPOIETIC SYSTEM
IONIZING RADIATIONS
MAMMALS
MICE
ORGANS
RADIATION EFFECTS
RADIATIONS
RODENTS
SOMATIC CELLS
STEM CELLS
TISSUES
TRANSPLANTS
VERTEBRATES
X RADIATION