Zinc and copper metabolism in embryonic turkey hepatocytes

Conference · Wed Mar 05 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6846604

Darcey, S E; Richards, M P; Klasing, K C; Steele, N C

Primary monolayer cultures of hepatocytes from turkey embryos (15-16 day old) were incubated in serum-free media fortified with varying levels of Zn or Cu. Cellular Zn increased 6-fold (0.25-1.53 ..mu..g/mg cell protein) with increasing media Zn (0-50 ..mu..M) and cellular Cu rose 57-fold (0.03 to 1.70 ..mu..g/mg) from 0-20 ..mu..M Cu. At 50 ..mu..M Zn for 24 hr, cellular Zn rose 4-fold (0.31 to 1.35 ..mu..g/mg) and cytosol Zn 6-fold (0.61 to 3.59 ..mu..g/mg cytosol protein). Metallothionein Zn from these cells (50 ..mu..M Zn, 24 hr) increased 10-fold (0.24 to 2.45 ..mu..g/mg cytosol protein) accounting for 68% of soluble Zn. At 20 ..mu..M Cu for 24 hr, cellular Cu increased 56-fold (0.03 to 1.67 ..mu..g/mg). During 24 hr in non-supplemented media, preloaded cells (50 ..mu..M Zn or 20 ..mu..M Cu), lost 50% of the accumulated Zn (1.05 to 0.46 ..mu..g/mg) and only 10% of the Cu (1.33 to 1.23 ..mu..g/mg). Synthesis of MT was determined in hepatocytes incubated with 10 ..mu..M Zn by the incorporation of /sup 65/Zn and /sup 35/S-cysteine. A 2-fold increase in /sup 65/Zn and an 8-fold increase in /sup 35/S-cysteine. A 2-fold increase in /sup 65/Zn and an 8-fold increase in /sup 35/S was observed in the Zn-treated cells. Addition of dexamethasone (dex) at 10/sup -7/M resulted in 1.14-and 1.85-fold increases in /sup 65/Zn and /sup 35/S incorporation, respectively. Combined, Zn and dex produced an additive effect. In conclusion, turkey embryo hepatocytes actively accumulate Zn and Cu in culture. A significant portion of Zn in the cytosol is bound to MT which is synthesized in response to added Zn and dex. Primary liver cell culture is a useful model to study hepatic metal metabolism during avian embryonic development.

• Research Organization: Dept. of Agriculture, Beltsville, MD
• OSTI ID: 6846604
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:4
• Country of Publication: United States
• Language: English

Related Subjects

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560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
BIOLOGICAL MODELS
BIRDS
COPPER
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTS
EMBRYONIC CELLS
EVEN-ODD NUCLEI
FOWL
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIVER CELLS
METABOLISM
METALS
NUCLEI
RADIOISOTOPES
SOMATIC CELLS
SULFUR 35
SULFUR ISOTOPES
TRACER TECHNIQUES
TRANSITION ELEMENTS
VERTEBRATES
ZINC
ZINC 65
ZINC ISOTOPES