Cu,Zn superoxide dismutase and copper deprivation and toxicity in Saccharomyces cerevisiae

Journal Article · Sun Dec 31 23:00:00 EST 1989 · Journal of Bacteriology; (USA)

OSTI ID:6742446

Greco, M A; Hrab, D I; Magner, W; Kosman, D J ^[1]

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1. State Univ. of New York, Buffalo (USA)

A wild-type strain of the yeast Saccharomyces cerevisiae grown at a medium (Cu)of {le}50 nM contained less Cu,Zn superoxide dismutase (SOD) mRNA (60%), protein (50%), and activity (50%) in comparison with control cultures grown in normal synthetic dextrose medium ((Cu) {approx} 150 nM). A compensating increase in the activity of MnSOD was observed, as well as a smaller increase in MnSOD mRNA. These medium (Cu)-dependent differences were observed in cultures under N{sub 2} as well. Addition of Cu{sup 2+} (100 {mu}M) to Cu-depleted cultures resulted in a rapid (30 min) increase in Cu,ZnSOD mRNA (2.5-fold), protein (3.5-fold), and activity (4-fold). Ethidium bromide (200 {mu}g/ml of culture) inhibited by 50% the increase in Cu,ZnSOD mRNA, while cycloheximide (100 {mu}g/ml of culture) inhibited completely the increase in protein and activity. Addition of Cu{sup 2+} to {ge}100 {mu}M caused no further increase in these parameters but did result in a loss of total cellular RNA and translatable RNA, a decline in the population of specific mRNAs, a decrease in total soluble protein and the activity of specific enzymes, and an inhibition of incorporation of ({sup 3}H)uracil and ({sup 3}H)leucine into trichloroacetic acid-insoluble material. Cu,ZnSOD mRNA, protein, and activity appeared relatively more resistant to these effects of Cu toxicity than did the other cellular constituents examined. When evaluated in cultures under N{sub 2}, the cellular response to (Cu) of {ge}100 {mu}M was limited to the inhibition of radiolabel incorporation into trichloroacetic acid-insoluble material. All other effects were absent in the absence of O{sub 2}.

• OSTI ID: 6742446
• Journal Information: Journal of Bacteriology; (USA), Journal Name: Journal of Bacteriology; (USA) Vol. 172:1; ISSN JOBAA; ISSN 0021-9193
• Country of Publication: United States
• Language: English

Related Subjects

550201 -- Biochemistry-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACIDS
AZINES
CARBOXYLIC ACIDS
COPPER
ELEMENTS
ENZYME ACTIVITY
ENZYMES
EUMYCOTA
FUNGI
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LEUCINE
MESSENGER-RNA
METALS
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDOREDUCTASES
PLANTS
PYRIMIDINES
RADICALS
RNA
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
SUPEROXIDE DISMUTASE
SUPEROXIDE RADICALS
TOXICITY
TRACER TECHNIQUES
TRANSCRIPTION
TRANSITION ELEMENTS
TRITIUM COMPOUNDS
URACILS
YEASTS