Expression of manganese and copper/zinc superoxide dismutase (MnSOD, Cu/ZnSOD) mRNA in cells from LPS-sensitive (LPS-s) and LPS-resistant (LPS-r) mice

Conference · Mon Mar 11 04:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5863313

Gibbs, L S; Del Vecchio, P J; Shaffer, J B ^[1]

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1. Albany Medical Coll., NY (United States)

Lipopolysaccharide (LPS) is thought to induce tissue damage through an oxidant-dependent mechanism mediated by cytokines such as tumor necrosis factor (TNF). Treatment of numerous cell types with TNF induces an increase in MnSOD, an antioxidant. To gain a further understanding of the relationship between LPS-induced injury and TNF and MnSOD induction, the authors examined the effect of LPS on MnSOD and CuZnSOD mRNA levels in cultured macrophages and lung endothelial cells (EC) derived from LPS-s and LPS-r mice. Macrophages from LPS-s and LPS-r mice treated with either 10 or 500 ng/ml LPS produced increased levels of MnSOD mRNA, although the magnitude of the increase was greater with the higher LPS dose. No changes in levels of Cu/ZnSOD mRNA were observed in macrophages. In contrast, lung EC MnSOD mRNA from LPS-s mice was induced to the same extent by either dose of LPS. Neither dose of LPS induced an appreciable increase in EC MnSOD mRNA from LPS-r mice, nor was an increase in MsSOD mRNA apparent when these cells were treated with TNF. EC Cu/ZnSOD mRNA levels were unaffected by LPS in macrophages compared to EC from LPS-r and LPS-s mice may be due to cell-specific responses to oxidative stress. Moreover, the LPS-induced increase in MnSOD mRNA in macrophages from LPS-r mice suggests that this inductive process may not be totally dependent on a coordinate increase in cytokine production.

• OSTI ID: 5863313
• Report Number(s): CONF-9104107--
• Conference Information: Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Journal Volume: 5:4
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
BODY
CARBOHYDRATES
CONNECTIVE TISSUE CELLS
COPPER
ELEMENTS
ENDOTHELIUM
ENZYMES
GROWTH FACTORS
KINETICS
LIPIDS
LIPOPOLYSACCHARIDES
LUNGS
LYMPHOKINES
MACROPHAGES
MAMMALS
MANGANESE
MESSENGER-RNA
METABOLISM
METALS
MICE
MITOGENS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
PHAGOCYTES
POLYSACCHARIDES
PROTEINS
REACTION KINETICS
RESPIRATORY SYSTEM
RNA
RODENTS
SACCHARIDES
SOMATIC CELLS
SUPEROXIDE DISMUTASE
SYNTHESIS
TISSUES
TRANSITION ELEMENTS
VERTEBRATES