Endotoxin treatment protects rats against ozone-induced lung edema: with evidence for the role of manganese superoxide dismutase

Journal Article · Sat Feb 29 23:00:00 EST 1992 · Toxicology and Applied Pharmacology; (United States)

DOI:https://doi.org/10.1016/0041-008X(92)90003-B· OSTI ID:5374324

Rahman, I; Massaro, D ^[1]

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1. Lung Biology Laboratory, Georgetown University School of Medicine, Washington, DC (United States)

Ozone is a strong oxidizing agent that can cause lung damage and edema. There is evidence that it does so by causing peroxidation of membrane lipids. However, the elevation in lung activity of copper, zinc superoxide dismutase (Cu, ZnSOD), and manganese superoxide dismutase (MnSOD) during exposure to ozone suggests that increased production of superoxide could contribute to lung edema caused by ozone. This latter observation, and preliminary evidence that treatment of rats with endotoxin elevates lung activity of MnSOD without elevation of the activity of Cu, ZnSOD, catalase (CAT), or glutathione peroxidase (GP), led to the present study. We treated rats with endotoxin, exposed them to different concentrations of ozone, measured lung wet weight to dry weight ratio, thiobarbituric acid-reactive material (TBAR), and assayed lung tissue for Cu, ZnSOD, MnSOD, CAT, and GP activity. Our major findings are, (1) a strongly edemogenic concentration of ozone-lowered MnSOD activity; (2) endotoxin treatment of air-breathing rats did not decrease lipid peroxidation as indicated by the lung concentration of TBAR; (3) induction of increased MnSOD activity in lung by treatment with endotoxin was associated with virtually complete protection against an otherwise edemogenic concentration of ozone, with less lipid peroxidation, and with less loss of weight; and (4) this protection occurred without elevated Cu, ZnSOD, CAT, or GP activity.

• OSTI ID: 5374324
• Journal Information: Toxicology and Applied Pharmacology; (United States), Journal Name: Toxicology and Applied Pharmacology; (United States) Vol. 113:1; ISSN TXAPA; ISSN 0041-008X
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
ANTIGENS
BIOLOGICAL EFFECTS
BIOLOGICAL FUNCTIONS
BODY
CELL CONSTITUENTS
CELL MEMBRANES
EDEMA
ENDOTOXINS
ENZYME ACTIVITY
ENZYMES
EXPOSURE CHAMBERS
HAZARDOUS MATERIALS
LIPIDS
LUNGS
MAMMALS
MATERIALS
MEMBRANES
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
OZONE
PATHOLOGICAL CHANGES
PROTEINS
RATS
RESPIRATORY SYSTEM
RODENTS
SUPEROXIDE DISMUTASE
SYMPTOMS
TOXIC MATERIALS
TOXICITY
TOXINS
VERTEBRATES