Zinc and dexamethasone induce metallothionein accumulation by endothelial cells

Conference · Mon Mar 11 04:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5371382

Briske-Anderson, M; Bobilya, D J; Reeves, P G ^[1]

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1. USDA/ARS, Grand Forks, ND (United States)

Several tissues increase their metallothionein (MT) concentration when exposed to elevated amounts of plasma Zn. Endothelial cells form the blood vessels that supply all tissues and constitute a barrier between cells of tissues and the blood. This study examined the ability of endothelial cells to synthesize MT and accumulate Zn in response to high amounts of Zn and dexamethasone. Bovine pulmonary endothelial cells were grown to confluence in Minimum Essential Medium with Earle's salts and 10% fetal calf serum. The monolayer was maintained for 2 d prior to use in medium containing EDTA-dialyzed serum. This low Zn medium was replaced with one containing 1, 6, 25, 50, 100, 150, or 200 {mu}M Zn and incubated for 24 hr before harvesting the cells. MT was quantified by the cadmium binding assay. Cellular Zn concentrations were analyzed by atomic absorption after a nitric acid digestion. The MT concentration was elevated in response to Zn concentrations of 100 {mu}M or more. Cellular Zn concentration was elevated when media Zn was 25 {mu}M or more. MT and cellular Zn concentrations were positively correlated. In another study, inclusion of 0.1 {mu}M dexamethasone in the media increased concentration at all Zn concentrations studied. However, the inclusion of 0.3 {mu}M cis-platinum had no effect. In conclusion, endothelial cells in culture respond to elevated amounts of Zn and dexamethasone in the media by accumulating Zn and MT.

• OSTI ID: 5371382
• Report Number(s): CONF-9104107--
• Conference Information: Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Journal Volume: 5:4
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ABSORPTION SPECTRA
ADRENAL HORMONES
ANIMAL TISSUES
ANIMALS
BIOLOGICAL ACCUMULATION
BIOLOGICAL EFFECTS
BIOLOGICAL FUNCTIONS
BODY
CORTICOSTEROIDS
DEXAMETHASONE
DOMESTIC ANIMALS
ELEMENTS
ENDOTHELIUM
GLUCOCORTICOIDS
HORMONES
HYDROXY COMPOUNDS
KETONES
LUNGS
MAMMALS
METALLOPROTEINS
METALLOTHIONEIN
METALS
ORGANIC COMPOUNDS
ORGANS
PREGNANES
PROTEINS
RESPIRATORY SYSTEM
RUMINANTS
SHEEP
SPECTRA
STEROID HORMONES
STEROIDS
TISSUES
VERTEBRATES
ZINC