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Regulation of tissue levels of metallothionein with emphasis on metallothionein degradation

Thesis/Dissertation ·
OSTI ID:6256747

The synthesis and degradation of metallothionein (MT) was studied in streptozotocin-induced diabetic rats and monolayer cultures of adult rat hepatocytes. Critical analysis of in vivo studies with diabetic rats and other literature revealed that cytoplasmic turnover of MT may not reflect actual degradation of this protein. Therefore, the characteristics of MT degradation in primary cultures of hepatocytes were investigated in subsequent studies. Hepatocytes were incubated in medium containing /sup 35/S-cysteine and 100 ..mu..M Zn overnight to induce MT synthesis. The level of /sup 35/S-MT was quantified in heat stable extracts of cell homogenates by Fast Protein Liquid Chromatography (FPLC). When Zn was removed from medium, the rate of /sup 35/S-MT turnover was found times faster than general /sup 3/H-protein. This decrease in cellular MT level reflected degradation since less than 1% of cellular MT was secreted. The rate of MT degradation was inversely proportional to cellular Zn status.

Research Organization:
Virginia Polytechnic Inst. and State Univ., Blacksburg (USA)
OSTI ID:
6256747
Country of Publication:
United States
Language:
English

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Related Subjects

550501* -- Metabolism-- Tracer Techniques
550901 -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOSYNTHESIS
CARBOXYLIC ACIDS
CHROMATOGRAPHY
CYSTEINE
DAYS LIVING RADIOISOTOPES
DIABETES MELLITUS
DISEASES
ELEMENTS
ENDOCRINE DISEASES
EVEN-ODD NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
LIQUID COLUMN CHROMATOGRAPHY
LIVER CELLS
MAMMALS
METABOLIC DISEASES
METABOLISM
METALLOPROTEINS
METALLOTHIONEIN
METALS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PATHOGENESIS
PROTEINS
RADIOISOTOPES
RATS
RODENTS
SEPARATION PROCESSES
SOMATIC CELLS
SULFUR 35
SULFUR ISOTOPES
SYNTHESIS
THIOLS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES
ZINC