Assessment of insulin action in insulin-dependent diabetes mellitus using (6(14)C)glucose, (3(3)H)glucose, and (2(3)H)glucose. Differences in the apparent pattern of insulin resistance depending on the isotope used
To determine whether (2(3)H), (3(3)H), and (6(14)C)glucose provide an equivalent assessment of glucose turnover in insulin-dependent diabetes mellitus (IDDM) and nondiabetic man, glucose utilization rates were measured using a simultaneous infusion of these isotopes before and during hyperinsulinemic euglycemic clamps. In the nondiabetic subjects, glucose turnover rates determined with (6(14)C)glucose during insulin infusion were lower (P less than 0.02) than those determined with (2(3)H)glucose and higher (P less than 0.01) than those determined with (3(3)H)glucose. In IDDM, glucose turnover rates measured with (6(14)C)glucose during insulin infusion were lower (P less than 0.05) than those determined with (2(3)H)glucose, but were not different from those determined with (3(3)H)glucose. All three isotopes indicated the presence of insulin resistance. However, using (3(3)H)glucose led to the erroneous conclusion that glucose utilization was not significantly decreased at high insulin concentrations in the diabetic patients. (6(14)C) and (3(3)H)glucose but not (2(3)H)glucose indicated impairment in insulin-induced suppression of glucose production. These results indicate that tritiated isotopes do not necessarily equally reflect the pattern of glucose metabolism in diabetic and nondiabetic man.
- Research Organization:
- Mayo Clinic, Rochester, MN
- OSTI ID:
- 6845645
- Journal Information:
- J. Clin. Invest.; (United States), Vol. 6
- Country of Publication:
- United States
- Language:
- English
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CARBON 14 COMPOUNDS
ISOTOPE EFFECTS
GLUCOSE
METABOLISM
STRUCTURE-ACTIVITY RELATIONSHIPS
TRITIUM COMPOUNDS
DIABETES MELLITUS
INSULIN
LIVER
ALDEHYDES
BODY
CARBOHYDRATES
DIGESTIVE SYSTEM
DISEASES
ENDOCRINE DISEASES
GLANDS
HEXOSES
HORMONES
LABELLED COMPOUNDS
METABOLIC DISEASES
MONOSACCHARIDES
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
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550501* - Metabolism- Tracer Techniques