Comparison of extracellular and net glucose oxidation measured isotopically and by indirect calorimetry during high and low glucose turnover

Journal Article · Wed May 01 00:00:00 EDT 1991 · American Journal of Clinical Nutrition; (United States)

OSTI ID:5556652

McMahon, M M; Marsh, H M; Rizza, R A ^[1]

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1. Mayo Clinic, Rochester, MN (USA)

To determine the extent to which glucose oxidation measured by indirect calorimetry reflects glucose oxidation measured isotopically, subjects were studied during a 6-h hyperinsulinemic euglycemic clamp (1 mU.kg-1.min-1) and during infusion of saline. (6-{sup 14}C)glucose was infused on both occasions. Breath was collected for determination of the specific activity of carbon dioxide, oxygen consumption, and carbon dioxide production. Glucose turnover during hyperinsulinemia was approximately eightfold higher than during saline infusion. During the final 1.5 h of the hyperinsulinemic glucose clamp, oxidation measured isotopically remained slightly but consistently lower (P less than 0.05) than that measured by indirect calorimetry (13.8 +/- 1.1 vs 16.5 +/- 1.7 mumol.kg-1.min-1, respectively). In contrast, during the saline infusion, glucose oxidation measured isotopically did not differ from that measured by indirect calorimetry (8.3 +/- 0.6 vs 7.2 +/- 2.8 mumol.kg-1.min-1, respectively). We conclude that although net glucose oxidation measured isotopically was slightly lower than that measured by indirect calorimetry, both techniques provide similar estimates of glucose oxidation over a wide range of glucose disposal.

• OSTI ID: 5556652
• Journal Information: American Journal of Clinical Nutrition; (United States), Journal Name: American Journal of Clinical Nutrition; (United States) Vol. 53:5; ISSN 0002-9165; ISSN AJCNA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
ANIMALS
CALORIMETRY
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CHALCOGENIDES
CHEMICAL REACTIONS
COMPARATIVE EVALUATIONS
GLUCOSE
HEXOSES
HORMONES
INSULIN
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MAN
METABOLISM
MONOSACCHARIDES
ORGANIC COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PEPTIDE HORMONES
PRIMATES
PROTEINS
REDOX REACTIONS
SACCHARIDES
TRACER TECHNIQUES
VERTEBRATES