Underestimation of glucose turnover corrected with high-performance liquid chromatography purification of (6- sup 3 H)glucose

Journal Article · Mon Jan 01 04:00:00 EST 1990 · American Journal of Physiology; (USA)

OSTI ID:7026904

Schwenk, W F; Butler, P C; Haymond, M W; Rizza, R A ^[1]

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1. Mayo Foundation, Rochester, MN (USA)

We have recently reported that during infusion of commercially available (6-3H)glucose, a radioactive nonglucose contaminant may accumulate in plasma causing errors in the measurement of glucose turnover. To determine whether purification of this tracer by HPLC (high-performance liquid chromatography) before infusion would eliminate the contaminant in plasma and remove the underestimation of glucose turnover reported during hyperinsulinemia, four normal subjects each underwent two 5-h euglycemic clamps during infusion of insulin (1 mU.kg-1.min-1). Glucose turnover was measured with either commercially available (6-3H)glucose or with HPLC-purified (6-3H)glucose. HPLC analysis of samples from the clamps done with commercially available (6-3H)glucose showed that 9.7% of the infused tracer and 26% of the plasma glucose 3H radioactivity were contaminants. In contrast, no contaminant was observed in the plasma during infusion of HPLC-purified (6-3H)glucose. During the last hour of the clamp, mean glucose turnover using commercially available (6-3H)glucose was less (P less than 0.01) than the mean glucose infusion rate (7.6 +/- 0.3 vs. 10.5 +/- 0.3 mg.kg-1.min-1) yielding apparent negative (P less than 0.001) hepatic glucose release. In contrast, when HPLC-purified (6-3H)glucose was employed, glucose turnover equaled the glucose infusion rate (10.4 +/- 0.9 vs. 10.2 +/- 0.9 mg.kg-1.min-1) and hepatic glucose release was no longer negative. We conclude that removal of a tritiated nonglucose contaminant in (6-3H)glucose by HPLC yields correct estimations of glucose turnover at steady state.

• OSTI ID: 7026904
• Journal Information: American Journal of Physiology; (USA), Journal Name: American Journal of Physiology; (USA) Vol. 258; ISSN 0002-9513; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
ANIMALS
CARBOHYDRATES
CHROMATOGRAPHY
DATA COVARIANCES
GLUCOSE
HEXOSES
HORMONES
HYDROGEN COMPOUNDS
INSULIN
ISOTOPE APPLICATIONS
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
MAN
METABOLISM
MONOSACCHARIDES
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PRIMATES
SACCHARIDES
SEPARATION PROCESSES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES