Effect of ozone on platelet-activating factor production in phorbol-differentiated HL60 cells, a human bronchial epithelial cell line (BEAS S6), and primary human bronchial epithelial cells

Journal Article · Sat Oct 31 23:00:00 EST 1992 · American Journal of Respiratory Cell and Molecular Biology; (United States)

DOI:https://doi.org/10.1165/ajrcmb/7.5.514· OSTI ID:6833546

Samet, J M; Noah, T L; Devlin, R B; Yankaskas, J R; McKinnon, K; Dailey, L A; Friedman, M ^[1]

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1. Univ. of North Carolina, Chapel Hill (United States)

Platelet-activating factor (PAF) is a phospholipid with a wide spectrum of pro-inflammatory properties. In the lung, PAF induces airway hyperresponsiveness, neutrophil sequestration, and increased vascular permeability. The alveolar macrophage and the bronchial epithelium are tissues that are exposed to inhaled ozone (O3). We studied the effect of an in vitro O3 exposure on PAF production in a macrophage-like HL60 human cell line (dHL60), a human bronchial epithelial cell line (BEAS S6), and also in primary human bronchial epithelial cells. PAF was quantified by thin-layer chromatographic separation of lipid extracts from cells radiolabeled with [3H]lysoPAF and by radioimmunoassay. In vitro exposure of dHL60 cells to 0.05 to 1.0 ppm O3 for 15 to 120 min was found to significantly increase PAF levels above air control values at all exposure levels and time points (average increase of 92%). Similarly, BEAS S6 cells grown on collagen-coated filter supports and exposed to 0.05 to 1.0 ppm O3 for 60 min released an average increase in PAF of 626% above control values. Primary human bronchial epithelial cells also demonstrated significant increases in [3H]PAF release (average increase of 289% after exposure to 1.0 ppm O3 for 60 min) compared with paired air controls. These findings suggest that some of the effects of O3 inhalation may be mediated by PAF.

• OSTI ID: 6833546
• Journal Information: American Journal of Respiratory Cell and Molecular Biology; (United States), Journal Name: American Journal of Respiratory Cell and Molecular Biology; (United States) Vol. 7:5; ISSN 1044-1549; ISSN AJRBEL
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALCOHOLS
ANIMAL TISSUES
ANIMALS
BIOASSAY
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BODY
BODY FLUIDS
BRONCHI
CELL CULTURES
CELL DIFFERENTIATION
CHROMATOGRAPHY
DIAGNOSTIC TECHNIQUES
EPITHELIUM
ESTERS
HUMAN POPULATIONS
HYDROXY COMPOUNDS
IMMUNOASSAY
IMMUNOLOGY
IN VITRO
INHALATION
INTAKE
ISOTOPE APPLICATIONS
LIPIDS
LUNGS
MAMMALS
MAN
MATERIALS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
OZONE
PHOSPHOLIPIDS
POPULATIONS
PRIMATES
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
RESPIRATORY SYSTEM
SEPARATION PROCESSES
THIN-LAYER CHROMATOGRAPHY
TISSUE CULTURES
TISSUES
TRACER TECHNIQUES
VERTEBRATES