In vitro ozone exposure increases release of arachidonic acid products from a human bronchial epithelial cell line

Journal Article · Sun Jan 31 23:00:00 EST 1993 · Toxicology and Applied Pharmacology; (United States)

DOI:https://doi.org/10.1006/taap.1993.1027· OSTI ID:6878124

McKinnon, K P; Madden, M C; Noah, T L; Devlin, R B ^[1]

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1. TRC Environmental Corporation, Chapel Hill, NC (United States)

Eicosanoids released after ozone exposure of a human bronchial epithelial cell line, BEAS-S6, were analyzed by high-pressure liquid chromatography (HPLC) of supernatants from exposed cells prelabeled with [3H]arachidonic acid. BEAS cells released thromboxane B2 (TxB2), prostaglandin E2 (PGE2), leukotriene C4 (LTC4), LTD4, LTE4, and 12-hydroxyheptadecatrienoic acid (HHT) after exposure to ozone at concentrations of 0.1, 0.25, 0.5, and 1.0 ppm. The eicosanoids were identified by coelution with authentic standards. The largest product from ozone-exposed BEAS cells was the most polar peak, designated Peak 1. Release of cyclooxygenase products such as TxB2, PGE2, and HHT was inhibited by acetylsalicylic acid. Peaks that migrated with authentic standards for LTB4, LTC4, and LTD4 were inhibited by the lipoxygenase inhibitor nordihydroguaiaretic acid. The leukotrienes LTB4 and LTC4/D4 could also be detected by immunoassay of concentrated peak fractions. Thus BEAS cells released eicosanoids from cyclooxygenase and lipoxygenase pathways of arachidonic acid metabolism following exposure to ozone. Airway epithelial cells may be an important source of eicosanoids following ozone stimulation in humans.

• OSTI ID: 6878124
• Journal Information: Toxicology and Applied Pharmacology; (United States), Journal Name: Toxicology and Applied Pharmacology; (United States) Vol. 118:2; ISSN TXAPA9; ISSN 0041-008X
• Country of Publication: United States
• Language: English

Related Subjects

550500 -- Metabolism
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACETYLSALICYLIC ACID
ACUTE EXPOSURE
ANALGESICS
ANIMAL CELLS
ANIMAL TISSUES
ANTIPYRETICS
ARACHIDONIC ACID
BIOASSAY
BIOLOGICAL EFFECTS
BODY
BRONCHI
CARBOXYLIC ACIDS
CENTRAL NERVOUS SYSTEM DEPRESSANTS
CHROMATOGRAPHY
DOSE-RESPONSE RELATIONSHIPS
DRUGS
EPITHELIUM
HYDROXY ACIDS
IMMUNOASSAY
IN VITRO
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
OZONE
RESPIRATORY SYSTEM
RESPIRATORY TRACT CELLS
SEPARATION PROCESSES
TISSUES