Arachidonic and eicosapentaenoic acid metabolism in bovine neutrophils and platelets: effect of calcium ionophore

Journal Article · Tue Sep 01 04:00:00 EDT 1987 · J. Leukocyte Biol.; (United States)

OSTI ID:6090072

Taylor, S M; Laegreid, W W; Heidel, J R; Straub, K M; Liggitt, H D; Silflow, R M; Breeze, R G; Leid, R W

Substitution of dietary fatty acids has potential for altering the inflammatory response. The purpose of the present study was to define the metabolites of arachidonic acid (AA) and eicosapentaenoic acid (EPA) secreted by bovine peripheral blood neutrophils and platelets. High performance liquid chromatography was used to characterize cyclooxygenase and lipoxygenase metabolites secreted in response to the calcium ionophore A23187. Cells were prelabelled with /sup 3/H-AA or /sup 3/H-EPA prior to challenge with the calcium ionophore. Bovine neutrophils secreted leukotriene B4 (LTB4) and 5-hydroxyeicosatetraenoic acid (5-HETE) as the major metabolites of AA, as well as the corresponding leukotriene B5 (LTB5) and 5-hydroxyeicosapentaenoic acid (5-HEPE) metabolites of EPA. Peptidoleukotrienes derived from /sup 3/H-AA or /sup 3/H-EPA were not detected under these conditions. The major tritiated metabolites secreted from bovine platelets were: thromboxane A2, measured as the stable metabolite thromboxane B2 (TXB2); hydroxyheptadecatrienoic acid (HHT) and 12-HETE derived from /sup 3/H-AA; and the omega-3 analogs TXB3 and 12-HEPE, derived from /sup 3/H-EPA. Preferred substrate specificities existed amongst the AA- and EPA-derived metabolites for the intermediary enzymes involved in the arachidonic acid cascade. These findings support the hypothesis that substitution of membrane-bound AA by EPA has potential for modulation of the host inflammatory response following cellular phospholipid mobilization.

• Research Organization: Washington State Univ., Pullman
• OSTI ID: 6090072
• Journal Information: J. Leukocyte Biol.; (United States), Journal Name: J. Leukocyte Biol.; (United States) Vol. 42:3; ISSN JLBIE
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH METALS
ANIMALS
ARACHIDONIC ACID
BIOLOGICAL FUNCTIONS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BODY FLUIDS
CALCIUM
CARBOXYLIC ACIDS
CATTLE
CHROMATOGRAPHY
DOMESTIC ANIMALS
EICOSANOIC ACID
ELEMENTS
FUNCTIONS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEUKOCYTES
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
MATERIALS
METABOLISM
METABOLITES
METALS
MONOCARBOXYLIC ACIDS
NEUTROPHILS
ORGANIC ACIDS
ORGANIC COMPOUNDS
RUMINANTS
SECRETION
SEPARATION PROCESSES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES