Production of metabolic products of arachidonic acid during cell-cell interactions
Interactions of human platelets and neutrophils were studied with particular reference to the arachidonic acid pathway. Suspensions of (3H)arachidonate-labeled platelets and unlabeled neutrophils were stimulated with ionophore A23187. We detected several radioactive arachidonate metabolites, which are not produced by platelets alone. These included (3H)-labeled leukotriene B4 (LTB4), dihydroxy-eicosatetraeonic acid (DiHETE), and 5-hydroxy-eicosatetraenoic acid (5-HETE). DiHETE was formed when the platelet product (3H)12-HETE was added to ionophore-stimulated neutrophils. In addition, DiHETE was the major metabolite when (3H)5-HETE, a neutrophil arachidonate product, was added to stimulated platelets. We therefore suggest that upon stimulation, platelet-derived arachidonate can serve as precursor for the neutrophil-derived eicosanoids LTB4 and 5-HETE, and the platelet-derived product 12-HETE can be metabolized to DiHETE by stimulated human neutrophils. More recently we have shown that 12-HETE from thrombin-stimulated platelets can also be metabolized to a new product, 12,20-DiHETE, by
- Research Organization:
- New York VA Medical Center, NY
- OSTI ID:
- 6269834
- Journal Information:
- J. Allergy Clin. Immunol.; (United States), Journal Name: J. Allergy Clin. Immunol.; (United States) Vol. 74:3; ISSN JACIB
- Country of Publication:
- United States
- Language:
- English
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