Effect of ozone on platelet activating factor synthesis in human bronchial epithelial cells

Conference · Mon Mar 11 04:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5265715

Samet, J M; Noah, T; McKinnon, K; Devlin, R; Friedman, M ^[1]

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1. Univ. of North Carolina, Chapel Hill (United States) ABB Environmental Services, Research Triangle Park, NC (United States) Environmental Protection Agency, Research Triangle Park, NC (United States)

Ozone (O{sub 3}) exposure results in lung inflammatory changes. Since the lung epithelium is a major target of O{sub 3}, the authors studied the effects of an in-vitro O{sub 3} exposure of a human bronchial epithelial cell line BEAS S6 on the synthesis of platelet activating factor (PAF), a potent lipid mediator. BEAS S6 cells were grown on collagen-coated filters and exposed in vitro, in duplicate, to air or O{sub 3} for 60 min. Following exposure, the conditioned media from the apical and basolateral compartments were assayed for PAF by radioimmunoassay. No change in viability, as {sup 51}Cr release, was found 24H after exposure in any group. A separate group of cells was labeled with 1 {mu}Ci/ml {sup 3}H-lysoPAF, exposed to 1.0 ppm O{sub 3} {times} 1H and the lipids in the cells and media extract and analyzed by thin layer chromatography. There was a 89% and 81% increase in PAF in the apical and basolateral media, respectively, and a 257% increase in intracellular PAF compared to air-exposed cells. These data are consistent with a role for epithelial-derived PAF in the response of the lung to O{sub 3}.

• OSTI ID: 5265715
• Report Number(s): CONF-9104107--
• Conference Information: Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Journal Volume: 5:4
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOASSAY
BIOSYNTHESIS
BLOOD COAGULATION FACTORS
BODY
BRONCHI
CHROMATOGRAPHY
CHROMIUM 51
CHROMIUM ISOTOPES
COAGULANTS
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
EPITHELIUM
EVEN-ODD NUCLEI
HEMATOLOGIC AGENTS
HYDROGEN COMPOUNDS
IMMUNOASSAY
IMMUNOLOGY
IN VITRO
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LUNGS
MAMMALS
MAN
NUCLEI
ORGANIC COMPOUNDS
ORGANS
OZONE
PRIMATES
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
RADIOISOTOPES
RESPIRATORY SYSTEM
SENSITIVITY
SEPARATION PROCESSES
SYNTHESIS
THIN-LAYER CHROMATOGRAPHY
TISSUES
TOXICITY
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES