BEAS S6 (BEAS) human bronchial epithelial cells produce inflammatory mediators following ozone(O sub 3 ) exposure

Conference · Mon Mar 11 04:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5264674

McKinnon, K; Joyce, M; Noah, T; Devlin, R; Koren, H ^[1]

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1. Univ. of North Carolina, Chapel Hill (United States) Environmental Protection Agency, Research Triangle Park, NC (United States)

Ozone induces an inflammatory response in humans. The purpose of this study was to assess the role of epithelial cells in this response. The authors exposed the bronchial epithelial cell line BEAS cultured on collagen-impregnated filters to air or varying concentrations of O{sub 3}. Fluid obtained from either apical or basolateral compartments was analyzed for various inflammatory mediators and other products released in response to O{sub 3} exposure. O{sub 3} had dose-dependent cytotoxic effects as determined by the release of LDH or {sup 51}Cr and by uptake of trypan blue or propidium iodide. PGE{sub 2}, leukotrienes C{sub 4}, B{sub 4}, and E{sub 4}, fibronectin, PAF, and IL 6 were produced in dose-dependent responses following exposure of BEAS cells to 0.1 to 1.0 ppm O{sub 3}. The data demonstrate that the BEAS cell line produces inflammatory substances in response to O{sub 3} and thus functions as a useful model to study pollutant/airway cell interactions.

• OSTI ID: 5264674
• Report Number(s): CONF-9104107--
• Conference Information: Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Journal Volume: 5:4
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL FUNCTIONS
BODY
BRONCHI
CHROMIUM 51
CHROMIUM ISOTOPES
DAYS LIVING RADIOISOTOPES
DOSE-RESPONSE RELATIONSHIPS
ELECTRON CAPTURE RADIOISOTOPES
EPITHELIUM
EVEN-ODD NUCLEI
GROWTH FACTORS
INFLAMMATION
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LYMPHOKINES
MAMMALS
MAN
MITOGENS
NUCLEI
ORGANIC COMPOUNDS
OZONE
PATHOLOGICAL CHANGES
PRIMATES
PROSTAGLANDINS
PROTEINS
RADIOISOTOPES
RESPIRATORY SYSTEM
SYMPTOMS
TISSUES
TOXICITY
TRACER TECHNIQUES
VERTEBRATES