The response of a human bronchial epithelial cell line to histamine: Intracellular calcium changes and extracellular release of inflammatory mediators

Journal Article · Fri Nov 01 04:00:00 EST 1991 · American Journal of Respiratory Cell and Molecular Biology; (United States)

DOI:https://doi.org/10.1165/ajrcmb/5.5.484· OSTI ID:5929363

Noah, T L; Paradiso, A M; Madden, M C; McKinnon, K P; Devlin, R B ^[1]

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1. Department of Pediatrics, University of North Carolina School of Medicine, Chapel Hill (United States)

Epithelial cells are likely to modulate inflammation and tissue repair in the airways, but the factors responsible for these processes remain unclear. Because human airway epithelia are infrequently available for in vitro studies, transformed epithelial cell lines are of interest as models. The authors therefore investigated the response of an SV-40/adenovirus-transformed human bronchial epithelial cell line (BEAS-2B) to histamine, a mediator with relevance for airway diseases. The intracellular calcium response to histamine (10(-4) M) was measured, using Fura-2 and microspectrofluorimetry. Histamine induced a transient increase in intracellular calcium that originated from intracellular sources; this effect was inhibited by the H1 receptor antagonist diphenhydramine, suggesting that BEAS cells retain functioning histamine receptors. BEAS cells were grown to confluence on microporous, collagen-coated filters, allowing measurement of vectorial release of soluble mediators. Monolayers exposed to histamine for 30 min released interleukin-6 and fibronectin in the apical direction, in a dose-dependent manner. Little eicosanoid production was induced by histamine, either in the apical or the basolateral direction, although BEAS cells constitutively produced small amounts of prostaglandin E2 and 15-HETE. However, these cells formed large amounts of eicosanoids in response to ozone exposure as a positive control. Comparison of their data with published reports for human airway epithelia in primary culture suggests that the BEAS cell line is, in a number of respects, a relevant model for the study of airway epithelial responses to a variety of stimuli.

• OSTI ID: 5929363
• Journal Information: American Journal of Respiratory Cell and Molecular Biology; (United States), Journal Name: American Journal of Respiratory Cell and Molecular Biology; (United States) Vol. 5:5; ISSN 1044-1549; ISSN AJRBE
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALINE EARTH METALS
AMINES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
AZOLES
BIOLOGICAL EFFECTS
BODY
BRONCHI
BRONCHITIS
CALCIUM
DISEASES
DOSE-RESPONSE RELATIONSHIPS
ELEMENTS
EPITHELIUM
HETEROCYCLIC COMPOUNDS
HISTAMINE
IMIDAZOLES
MAMMALS
MAN
MEMBRANE PROTEINS
METALS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OZONE
PRIMATES
PROTEINS
RECEPTORS
RESPIRATORY SYSTEM
RESPIRATORY SYSTEM DISEASES
SENSITIVITY
TISSUES
VERTEBRATES