Immune-related intestinal Cl sup minus secretion. I. Effect of histamine on the T sub 84 cell line
- Univ. of California, San Diego (USA)
The mast cell mediator, histamine, induces a rapid and transient increase in chloride secretion across monolayers of the human colonic epithelial cell line, T{sub 84}. Threshold stimulation occurred at 3 x 10{sup {minus}6} M histamine and a maximal effect at 10{sup {minus}4} M. The effect was reproduced by the H{sub 1} agonists 2-methylhistamine and 2-pyridylethylamine, but not by the H{sub 2} agonists 4-methylhistamine and dimaprit, suggesting the involvement of an H{sub 1} receptor. Additionally, histamine's action was inhibited by an H{sub 1} antagonist, diphenhydramine, but not by an H{sub 2} antagonist, cimetidine. Histamine treatment increased free cytosolic calcium levels, but not those of adenosine 3{prime},5{prime}-cyclic monophosphate (cAMP) or guanosine 3{prime},5{prime}-cyclic monophosphate (cGMP). The mechanism of chloride secretion induced by histamine resembled that of carbachol, in that both (1) were associated with an increase in free cytosolic calcium, (2) had a site of activation at a basolaterally localized K{sup +} channel, and (3) were potentiated by both cAMP- and cGMP-mediated secretagogues. These results suggest that histamine may act as an intestinal secretagogue via direct interactions with epithelial cells.
- OSTI ID:
- 7191774
- Journal Information:
- American Journal of Physiology; (USA), Journal Name: American Journal of Physiology; (USA) Vol. 254:1; ISSN 0002-9513; ISSN AJPHA
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL ISOTOPES
AMINES
ANIMAL CELLS
AZOLES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BODY
CHLORINE 36
CHLORINE ISOTOPES
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DOSE-RESPONSE RELATIONSHIPS
ELECTRON CAPTURE RADIOISOTOPES
GASTROINTESTINAL TRACT
HETEROCYCLIC COMPOUNDS
HISTAMINE
IMIDAZOLES
INTERMEDIATE MASS NUCLEI
INTESTINES
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
KINETICS
LIGHT NUCLEI
MAST CELLS
MEMBRANE TRANSPORT
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
RADIOISOTOPES
REACTION KINETICS
RUBIDIUM 86
RUBIDIUM ISOTOPES
SECRETION
SODIUM 22
SODIUM ISOTOPES
SOMATIC CELLS
YEARS LIVING RADIOISOTOPES