Effects of ozone exposure on lipid metabolism in human alveolar macrophages
Alveolar macrophages (AM) store arachidonic acid (AA) which is esterified in cellular phospholipids until liberated by phospholipase A2 or C after exposure to inflammatory stimuli. Following release, there can be subsequent metabolism of AA into various potent, biological active mediators including prostaglandins and platelet activating factor (PAF). To examine the possibility that these mediators may account for some of the pathophysiologic alterations seen in the lung following O3 exposure, human AM were collected by bronchoalveolar lavage of normal subjects, plated into tissue culture dishes, and the adherent cells were incubated with 3H-AA or 3H-lysoPAF. Human AM exposed 1.0 ppm O3 for 2 hr released 65 + or - 12% more tritium, derived from 3H-AA, than paired air-exposed controls into media supernatants. In other studies using a similar O3 exposure protocol, there was also a significant increase in human AM PGE2 production (2.0 + or - 0.5 fold-increase above air-exposure values, p<0.01, n=17). In additional studies, using a similar O3 exposure protocol (1.0 ppm for 1 hr), there was also a significant increase in human AM PAF content (1.7 + or - 0.2 fold-increase above air-exposure values, p<0.02, n=5).
- Research Organization:
- North Carolina Univ., Chapel Hill, NC (United States). Center for Environmental Medicine and Lung Biology
- OSTI ID:
- 5600438
- Report Number(s):
- PB-91-176982/XAB; CNN: EPA-R-812738
- Country of Publication:
- United States
- Language:
- English
Similar Records
Effect of ozone on platelet-activating factor production in phorbol-differentiated HL60 cells, a human bronchial epithelial cell line (BEAS S6), and primary human bronchial epithelial cells
The effect of ozone exposure on rat alveolar macrophage arachidonic acid metabolism
Related Subjects
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ARACHIDONIC ACID
BIOLOGICAL EFFECTS
BLOOD COAGULATION FACTORS
BODY
CARBOXYLIC ACIDS
COAGULANTS
CONNECTIVE TISSUE CELLS
DRUGS
HEMATOLOGIC AGENTS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
LAVAGE
LIPIDS
LUNGS
MACROPHAGES
MAMMALS
MAN
METABOLISM
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OZONE
PHAGOCYTES
PRIMATES
PROSTAGLANDINS
PROTEINS
RESPIRATORY SYSTEM
SOMATIC CELLS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES