Effects of ozone exposure on lipid metabolism in human alveolar macrophages
- Univ. of North Carolina, Chapel Hill, NC (United States)
- Environmental Protection Agency, Research Triangle Park, NC (United States)
Alveolar macrophages (AM) store arachidonic acid (AA), which is esterified in cellular phospholipids until liberated by phospholipase A[sub C] or C after exposure to inflammatory stimuli. After release, there can be subsequent metabolism of AA into various potent, biologically active mediators including prostaglandins and platelet-activating factor (PAF). To examine the possibility that these mediators may account for some of the pathophysiologic alterations seen in the lung after ozone (O[sub 3]) exposure, human AM were collected by bronchoalveolar lavage of normal subjects, plated into tissue culture dishes, and the adherent cells were incubated with [[sup 3]H]AA or [[sup 3]H]lysoPAF. Human AM exposed to 1.0 ppm O[sub 3] for 2 hr released 65 [+-] 12% more tritium, derived from [[sup 3]H]AA, than paired, air-exposed controls into media supernatants. In other studies using a similar O[sub 3] exposure protocol, there was also a significant increase in human AM prostaglandin E[sub 2] production (2.0 [+-] 0.5-fold increase above air-exposure values, p < 0.02, n = 5). These potent lipid mediators, originally derived from human AM, may play an important role in the mechanisms of O[sub 3] lung toxicity. 25 refs., 2 figs., 5 tabs.
- OSTI ID:
- 6792949
- Journal Information:
- Environmental Health Perspectives; (United States), Journal Name: Environmental Health Perspectives; (United States) Vol. 97; ISSN EVHPAZ; ISSN 0091-6765
- Country of Publication:
- United States
- Language:
- English
Similar Records
Effect of ozone on platelet activating factor synthesis in human bronchial epithelial cells
The effect of ozone exposure on rat alveolar macrophage arachidonic acid metabolism
Related Subjects
550500 -- Metabolism
560300 -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ARACHIDONIC ACID
BIOLOGICAL PATHWAYS
BODY
CARBOXYLIC ACIDS
CONNECTIVE TISSUE CELLS
DYNAMIC FUNCTION STUDIES
ESTERS
LIPIDS
LUNGS
MACROPHAGES
METABOLIC ACTIVATION
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
OZONE
PHAGOCYTES
PHOSPHOLIPIDS
PROSTAGLANDINS
RESPIRATORY SYSTEM
SOMATIC CELLS
TOXICITY