Pulsed laser fluorometry for environmental monitoring
A compact pulsed laser fluorometer has been incorporated into a continuous flow system developed to detect acetylcholinesterase (AChE) inhibitors and/or primary amine compounds in air and water. A pulsed nitrogen laser pumped dye laser excites fluorescent reactants which flow continuously through a quartz flow cell. Data are collected, analyzed, and displayed using a Macintosh II personal computer. For detection of cholinesterase inhibitors the fluorogenic substrate N methylindoxyl acetate is used to monitor the activity of immobilized enzyme. Presence of inhibitors results in a decrease of steady state fluorescence. Detection of compounds containing primary amines is based on their reaction with fluorescamine to rapidly produce intensely fluorescent products. Compounds of interest to our research were amino acids, peptides, and proteins. An increase in steady state fluorescence could be cause to evaluate the reasons for the change. The detection limit of the protein, bovine serum albumin (BSA) in water is 10 ppT. Nebulized BSA concentrated by the LANL air sampler can be detected at sub ppT original air concentration. 16 refs., 14 figs., 3 tabs.
- Research Organization:
- Los Alamos National Lab., NM (USA)
- Sponsoring Organization:
- DOE/MA; DOS
- DOE Contract Number:
- W-7405-ENG-36
- OSTI ID:
- 6811209
- Report Number(s):
- LA-UR-90-2075; CONF-9006219--1; ON: DE90013163
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY
54 ENVIRONMENTAL SCIENCES
540120* -- Environment
Atmospheric-- Chemicals Monitoring & Transport-- (1990-)
540320 -- Environment
Aquatic-- Chemicals Monitoring & Transport-- (1990-)
ACETYLCHOLINE
AIR
ALBUMINS
AMINES
AMINO ACIDS
AMMONIUM COMPOUNDS
AUTONOMIC NERVOUS SYSTEM AGENTS
CARBOXYLESTERASES
CARBOXYLIC ACIDS
CELL FLOW SYSTEMS
CHOLINESTERASE
DETECTION
DRUGS
ENVIRONMENT
ENZYME INHIBITORS
ENZYMES
ESTERASES
ESTERS
FLUIDS
FLUORESCENCE
GASES
HYDROGEN COMPOUNDS
HYDROLASES
LASERS
LUMINESCENCE
MONITORING
NEUROREGULATORS
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PARASYMPATHOMIMETICS
PEPTIDES
PROTEINS
QUATERNARY COMPOUNDS
SAMPLING
WATER