A Naked-Eye Visible and Fluorescence 'Turn-On' Probe for Acetylcholinesterase Assay and the Discrimination of Biothiols
Journal Article
·
· Journal of Applied Spectroscopy
- Xi’an Medical College (China)
- Beijing Research Center of Agricultural Standards and Testing (China)
- Shaanxi Normal University, School of Chemistry and Chemical Engineering (China)
We demonstrate the utility of 4-chloro-7-nitrobenzofurazan (NBD-Cl), a simple, low-cost probe, for colorimetrically and fluorescently detecting and differentiating similar thiol species, including cysteine, homocysteine, and glutathione, as well as for assaying acetylcholinesterase (AChE) activity. This assay is a potential tool for investigating thiol functions in disease and diagnosis, and for screening AChE inhibitors. We envision that this versatile probe, which is commercially available and inexpensive, will be useful for further elucidating the roles of thiols in biology and also shows applicability for analysis of AChE inhibitors in physiological samples.
- OSTI ID:
- 22809975
- Journal Information:
- Journal of Applied Spectroscopy, Journal Name: Journal of Applied Spectroscopy Journal Issue: 3 Vol. 85; ISSN 0021-9037
- Country of Publication:
- United States
- Language:
- English
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