A simple radiometric in vitro assay for acetylcholinesterase inhibitors
Journal Article
·
· J. Pharm. Sci.; (United States)
A radiometric method for screening acetylcholinesterase inhibitors has been described. The method is based on the production of (/sup 14/C)carbon dioxide from the hydrolysis of acetylcholine. The inhibitory concentration at 50% (IC50) values for several known acetylcholinesterase inhibitors were in agreement with literature values. The new radiometric method is simple, inexpensive, and has the potential for automation.
- Research Organization:
- Department of Environmental Health Sciences, Johns Hopkins Medical Institutions, Baltimore, MD
- OSTI ID:
- 5821944
- Journal Information:
- J. Pharm. Sci.; (United States), Journal Name: J. Pharm. Sci.; (United States) Vol. 72:1; ISSN JPMSA
- Country of Publication:
- United States
- Language:
- English
Similar Records
A Naked-Eye Visible and Fluorescence 'Turn-On' Probe for Acetylcholinesterase Assay and the Discrimination of Biothiols
Synthesis, in vitro assays, molecular docking, theoretical ADMET prediction, and evaluation of 4-methoxy-phenylthiazole-2-amine derivatives as acetylcholinesterase inhibitors
Concentration-dependent interactions of the organophosphates chlorpyrifos oxon and methyl paraoxon with human recombinant acetylcholinesterase
Journal Article
·
Sun Jul 15 00:00:00 EDT 2018
· Journal of Applied Spectroscopy
·
OSTI ID:22809975
Synthesis, in vitro assays, molecular docking, theoretical ADMET prediction, and evaluation of 4-methoxy-phenylthiazole-2-amine derivatives as acetylcholinesterase inhibitors
Journal Article
·
Tue Oct 01 00:00:00 EDT 2019
· Medicinal Chemistry Research (Print)
·
OSTI ID:22936169
Concentration-dependent interactions of the organophosphates chlorpyrifos oxon and methyl paraoxon with human recombinant acetylcholinesterase
Journal Article
·
Fri Jun 01 00:00:00 EDT 2007
· Toxicology and Applied Pharmacology
·
OSTI ID:20976952
Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACETYLCHOLINE
AMINES
AMMONIUM COMPOUNDS
AUTONOMIC NERVOUS SYSTEM AGENTS
CARBON 14 COMPOUNDS
CARBOXYLESTERASES
CHOLINESTERASE
DRUGS
ENZYME INHIBITORS
ENZYMES
ESTERASES
ESTERS
HYDROLASES
IN VITRO
LABELLED COMPOUNDS
NEUROREGULATORS
ORGANIC COMPOUNDS
PARASYMPATHOMIMETICS
QUATERNARY COMPOUNDS
RADIOASSAY
59 BASIC BIOLOGICAL SCIENCES
ACETYLCHOLINE
AMINES
AMMONIUM COMPOUNDS
AUTONOMIC NERVOUS SYSTEM AGENTS
CARBON 14 COMPOUNDS
CARBOXYLESTERASES
CHOLINESTERASE
DRUGS
ENZYME INHIBITORS
ENZYMES
ESTERASES
ESTERS
HYDROLASES
IN VITRO
LABELLED COMPOUNDS
NEUROREGULATORS
ORGANIC COMPOUNDS
PARASYMPATHOMIMETICS
QUATERNARY COMPOUNDS
RADIOASSAY