Involvement of endogenous opiates in regulation of gastric emptying of fat test meals in mice

Journal Article · Mon Aug 01 04:00:00 EDT 1988 · Am. J. Physiol.; (United States)

OSTI ID:6805249

Fioramonti, J; Fargeas, M J; Bueno, L

The role of endogenous opioids and cholecystokinin (CCK) in gastric emptying was investigated in mice killed 30 min after gavage with /sup 51/Cr-radiolabeled liquid meals. The meals consisted of 0.5 ml of milk or one of five synthetic meals containing arabic gum, glucose and/or arachis oil and/or casein. Naloxone (0.1 mg/kg sc) significantly (P less than 0.01) accelerated gastric emptying of milk and meals containing fat but did not modify gastric emptying of nonfat meals. The CCK antagonist asperlicin (0.1 mg/kg ip) increased by 25% gastric emptying of milk. The gastric emptying of meals containing glucose and casein but not fat was reduced after administration of the COOH-terminal octapeptide of cholecystokinin (CCK-8, 4 micrograms/kg ip). This decrease was antagonized by both asperlicin (10 mg/kg ip) and naloxone (0.1 mg/kg sc). Intracerebroventricular (icv) administration of an opiate antagonist that poorly crosses the blood-brain barrier, methyl levallorphan (10 micrograms/kg), did not modify gastric emptying of milk but accelerated it when peripherally administered (0.1 mg/kg sc). Similarly, asperlicin (icv) administered at a dose of 1 mg/kg did not affect milk emptying. These results indicate that endogenous opiates are involved at peripheral levels in the regulation of gastric emptying of fat meals only and that such regulation involves release of CCK.

• Research Organization: Institut National de la Recherche Agronomique, Toulouse (France)
• OSTI ID: 6805249
• Journal Information: Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States) Vol. 255; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BETA DECAY RADIOISOTOPES
BIOLOGICAL FUNCTIONS
BIOLOGICAL MATERIALS
BODY FLUIDS
CARBOHYDRATES
CASEIN
CENTRAL NERVOUS SYSTEM DEPRESSANTS
CHROMIUM 51
CHROMIUM ISOTOPES
DIET
DIGESTION
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ENDORPHINS
EVEN-ODD NUCLEI
FATS
FOOD
FUNCTIONS
GLUCOSE
HEXOSES
HOMEOSTASIS
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
KININS
MAMMALS
MATERIALS
METHADONE HYDROCHLORIDE
MICE
MILK
MONOSACCHARIDES
NARCOTICS
NEUROREGULATORS
NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PEPTIDES
POLYPEPTIDES
PROTEINS
RADIOISOTOPES
RESPONSE MODIFYING FACTORS
RODENTS
SACCHARIDES
TRACER TECHNIQUES
VERTEBRATES