Characterization of specific /sup 125/I-bolton hunter (BH)-CCK-8 binding in guinea pig stomach muscle membranes - similar to pancreas but different from gastric glands

Conference · Fri Feb 28 23:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5277980

Chang, R S.L.; Lotti, V J; Chen, T B; Kunkel, K K

Cholecystokinin (CCK) is a gastrointestinal hormone which regulates gastric motility. The authors have characterized specific /sup 125/I-BH-CCK-8 binding in guinea pig stomach muscle membranes. The relative potencies of various CCK related peptides to inhibit specific /sup 125/I-CCK-8 binding in stomach muscle membranes resembled their rank order determined for pancreatic CCK receptor binding (CCK-8>> CCK-8-desulfate = gastrin CCK-4). The specific peripheral CCK antagonist, asperlicin and other non- selective CCK antagonists including proglumide, CBZ-CCK (26-32) amide, dibutyrylcyclic GMP and benzotript effectively inhibited /sup 125/I-CCK-8 binding in gastric muscle membranes with IC/sub 50/'s comparable to those for pancreatic CCK receptors. In contrast, specific /sup 125/I-CCK-8 binding in guinea pig gastric glands was inhibited equally well by gastrin and CCK-8. Moreover, asperlicin did not inhibit /sup 125/I-CCK-8 binding in guinea gastric glands. These results indicate CCK receptors in the stomach muscle membranes are similar to pancreatic CCK receptors but different from brain CCK or gastrin receptors.

• Research Organization: Merck Sharp and Dohme Research Labs., West Point, PA
• OSTI ID: 5277980
• Report Number(s): CONF-8604222-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:3; ISSN FEPRA
• Country of Publication: United States
• Language: English

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550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BODY
CELL CONSTITUENTS
CELL MEMBRANES
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ELECTRON CAPTURE RADIOISOTOPES
GASTROINTESTINAL TRACT
GUINEA PIGS
HORMONES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
MUSCLES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
POLYPEPTIDES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
RODENTS
STOMACH
TRACER TECHNIQUES
VERTEBRATES