Cholecystokinin receptor mediated hydrolysis of inositol phospholipids in guinea pig gastric glands
CCK-octapeptide (CCK-8)(EC/sub 50/ = 0.5 nM), in the presence of Li/sup +/, increased /sup 3/H-inositol phosphate (IP) accumulation in guinea pig gastric glands prelabeled with /sup 3/H-inositol. CCK-8 desulfate, human gastrin I and pentagastrin were much less potent than CCK-8. Antagonists of CCK receptors such as proglumide, dibutyryl-c-GMP and CBZ-Tyr(SO/sub 3/H)-Met-Gly-Trp-Met-AspNH/sup 2/ shifted the CCK dose response curve to the right. However, histamine (H/sub 1/ and H/sub 2/), cholinergic, substance P and ..cap alpha..- and ..beta..-adrenergic receptor antagonists had no effect on /sup 3/H-IP accumulation induced by CCK. The results suggest that CCK receptor activation in gastric glands leads to an enhanced breakdown of inositol phospholipids which may relate to calcium mobilization and pepsinogen secretion. 20 references, 2 figures, 1 table.
- Research Organization:
- Merck Sharp and Dohme Research Labs., West Point, PA
- OSTI ID:
- 5476846
- Journal Information:
- Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 36:10; ISSN LIFSA
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH METALS
AMINES
ANIMALS
AZOLES
BODY
CALCIUM
CARBOHYDRATES
CHEMICAL REACTIONS
DECOMPOSITION
DIGESTIVE SYSTEM
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ELEMENTS
ENDOCRINE GLANDS
ESTERS
GLANDS
GUINEA PIGS
HETEROCYCLIC COMPOUNDS
HISTAMINE
HORMONE ANTAGONISTS
HYDROLYSIS
IMIDAZOLES
INOSITOL
INOSITOLS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPIDS
LYSIS
MAMMALS
METALS
MONOSACCHARIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PANCREAS
PEPTIDES
PHOSPHOLIPIDS
PROTEINS
RECEPTORS
RODENTS
SACCHARIDES
SOLVOLYSIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES