Characterization of gastrin receptors on guinea pig pancreatic acini

Journal Article · Mon Nov 30 23:00:00 EST 1987 · American Journal of Physiology; (USA)

OSTI ID:6994877

Yu, Dahong; Noguchi, Masato; Zhou, Zhichao; Villanueva, M L; Gardner, J D; Jensen, R T ^[1]

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1. National Institutes of Health, Bethesda, MD (USA)

Recent studies have demonstrated gastrin receptors in some pancreatic tumors and that gastrin is a potent stimulant of pancreatic Na{sup +}-H{sup +} exchange. In the present study the authors used {sup 125}I-labeled gastrin ({sup 125}I-gastrin) to characterize gastrin receptors on guinea pig pancreatic acini. Binding of {sup 125}I-gastrin was temperature dependent, saturable, and specific for gastrin-related peptides. Analysis demonstrated a single class of receptors with high affinity for gastrin and a binding capacity of 1 fmol/mg protein. Binding of {sup 125}I-gastrin was inhibited with the following relative potencies: cholecystokinin octapeptide (CCK-8) > gastrin-17-I = gastrin-34-I > pentagastrin > desulfated (des(SO{sub 3}))CCK-8 > CCK-4 and by the receptor antagonists CBZ-CCK-27-32-NH{sub 2} > proglumide analogue 10 > asperlicin > Bt{sub 2}-guanosine 3{prime},5{prime}-cyclic monophosphate. The present results demonstrate that guinea pig pancreatic acini possess gastrin receptors that have a high affinity for gastrin and are distinct from CCK receptors previously described. Only occupation of the CCK receptors results in enzyme secretion. Gastrin receptors on pancreatic acini resemble those described in parietal cells and gastric glands; however, their function is unknown.

• OSTI ID: 6994877
• Journal Information: American Journal of Physiology; (USA), Journal Name: American Journal of Physiology; (USA) Vol. 253:6; ISSN 0002-9513; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH ISOTOPES
AMYLASE
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
BODY
CALCIUM 45
CALCIUM ISOTOPES
CHEMISTRY
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DOSE-RESPONSE RELATIONSHIPS
ELECTRON CAPTURE RADIOISOTOPES
ENDOCRINE GLANDS
ENZYMES
EVEN-ODD NUCLEI
GASTRIN
GLANDS
GLYCOSYL HYDROLASES
GUINEA PIGS
HORMONES
HYDROLASES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KININS
MAMMALS
MEMBRANE PROTEINS
MEMBRANE TRANSPORT
NUCLEI
O-GLYCOSYL HYDROLASES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PANCREAS
PEPTIDE HORMONES
PEPTIDES
POLYPEPTIDES
PROTEINS
RADIOISOTOPES
RADIORECEPTOR ASSAY
RECEPTORS
RODENTS
TEMPERATURE DEPENDENCE
TRACER TECHNIQUES
VERTEBRATES