Asperlicin antagonizes stimulatory effects of cholecystokinin on isolated islets

Journal Article · Sat Feb 28 23:00:00 EST 1987 · Am. J. Physiol.; (United States)

OSTI ID:5437214

Zawalich, W S; Diaz, V A

Asperlicin, a product derived from the fungus Aspergillus alliaceus, antagonized the multiple stimulatory effects of cholecystokinin (CCK-8S) on isolated islets. At a level of 10 uM, asperlicin completely inhibited insulin release in response to 25 nM CCK-8S. Increasing the level of CCK-8S to 100 nM partially restored a secretory response, while an even greater insulin stimulatory effect was noted with 500 nM CCK-8S. The inhibitory effect of asperlicin on CCK-8S-induced release was reversible. Asperlicin exposure had no effect on glucose or glyceraldehyde-induced secretion. Asperlicin reduced, in parallel with secretion, the increase in /sup 3/H efflux from (/sup 3/H) inositol prelabeled islets usually noted with CCK-8S addition. Asperlicin did not influence the small glucose-stimulated increase in /sup 3/H efflux. The results support the notion that asperlicin is a specific and potent antagonist of the multiple stimulatory effects of CCK-8S on islet tissue.

• Research Organization: Yale Univ. School of Nursing, New Haven, CT
• OSTI ID: 5437214
• Journal Information: Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States) Vol. 252:3; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ASPERGILLUS
BIOLOGICAL EFFECTS
BODY
CARBOHYDRATES
DIGESTIVE SYSTEM
DRUGS
ENDOCRINE GLANDS
FUNGI
GLANDS
GLUCOSE
HEXOSES
INOSITOLS
KININS
LABELLED COMPOUNDS
MONOSACCHARIDES
ORGANIC COMPOUNDS
ORGANS
PANCREAS
PEPTIDES
PLANTS
POLYPEPTIDES
PROTEINS
SACCHARIDES
SECRETION
TRITIUM COMPOUNDS