Effect of cholecystokinin on the accumulation of inositol phosphates in isolated pancreatic islets

Journal Article · Fri Dec 01 04:00:00 EST 1989 · American Journal of Physiology; (USA)

OSTI ID:6983960

Florholmen, J; Malm, D; Vonen, B; Burhol, P G ^[1]

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1. University Hospital of Tromso (Norway)

Sulfated cholecystokinin octapeptide (CCK-8S) potentiated glucose-induced secretion in isolated pancreatic islets with a maximal effect at 12 mM glucose, whereas no effect was observed at 3.3 and 25 mM glucose. This effect of CCK-8S was maximal at 10(-7) M. Anion-exchange fast-protein liquid chromatography analysis of (3H)inositol phosphates derived from islets prelabeled with myo-(3H)inositol showed that glucose induced accumulation of the 1,4,5-isomer of inositol trisphosphate and of inositol tetrakisphosphate. At 3.3 mM glucose, CCK-8S stimulated accumulation of inositol trisphosphate and inositol tetrakisphosphate to levels induced by 25 mM glucose alone. The net effect of CCK-8S on the accumulation of the inositol phosphates was maximal at 12 mM glucose and decreased at higher glucose concentrations. At 12 mM glucose the accumulation of inositol phosphates increased gradually up to 10(-7) M CCK-8S. This study indicates that CCK-8S potentiates glucose-induced insulin secretion through a mechanism involving the hydrolysis of polyphosphoinositides and the generation of inositol phosphates. However, activation of the inositol cycle per se did not seem to induce insulin secretion.

• OSTI ID: 6983960
• Journal Information: American Journal of Physiology; (USA), Journal Name: American Journal of Physiology; (USA) Vol. 257; ISSN 0002-9513; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
BODY
CARBOHYDRATES
CHROMATOGRAPHY
DIGESTIVE SYSTEM
ENDOCRINE GLANDS
ESTERS
GLANDS
GLUCOSE
HEXOSES
HORMONES
HYDROGEN COMPOUNDS
IN VITRO
INSULIN
ION EXCHANGE CHROMATOGRAPHY
ISOTOPE APPLICATIONS
ISOTOPE DILUTION
KINETICS
KININS
LIPIDS
MAMMALS
MONOSACCHARIDES
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PANCREAS
PEPTIDE HORMONES
PEPTIDES
PHOSPHOLIPIDS
POLYPEPTIDES
PROTEINS
RATS
REACTION KINETICS
RODENTS
SACCHARIDES
SECRETION
SEPARATION PROCESSES
SYNTHESIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES