Modulation of agonist-induced inositol phosphate metabolism by cyclic adenosine 3',5'-monophosphate in adrenal glomerulosa cells

Journal Article · Thu Nov 01 04:00:00 EST 1990 · Molecular Endocrinology; (USA)

DOI:https://doi.org/10.1210/mend-4-11-1712· OSTI ID:6056498

Baukal, A J; Hunyady, L; Balla, T; Ely, J A; Catt, K J ^[1]

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1. National Institute of Child Health and Human Development, Bethesda, MD (USA)

Activation of the cAMP messenger system was found to cause specific changes in angiotensin-II (All)-induced inositol phosphate production and metabolism in bovine adrenal glomerulosa cells. Pretreatment of (3H)inositol-labeled glomerulosa cells with 8-bromo-cAMP (8Br-cAMP) caused both short and long term changes in the inositol phosphate response to stimulation by All. Exposure to 8Br-cAMP initially caused dose-dependent enhancement (ED50 = 0.7 microM) of the stimulatory action of All (50 nM; 10 min) on the formation of D-myo-inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) and its immediate metabolites. This effect of 8Br-cAMP was also observed in permeabilized (3H)inositol-labeled glomerulosa cells in which degradation of Ins(1,4,5)P3 was inhibited, consistent with increased activity of phospholipase-C. Continued exposure to 8Br-cAMP for 5-16 h caused selective enhancement of the All-induced increases in D-myo-inositol 1,3,4,6-tetrakisphosphate (Ins(1,3,4,6)P4) and myo-inositol 1,4,5,6-tetrakisphosphate. The long term effect of 8Br-cAMP on the 6-phosphorylated InsP4 isomers, but not the initial enhancement of Ins(1,4,5)P3 formation, was inhibited by cycloheximide. The characteristic biphasic kinetics of All-induced Ins(1,4,5)P3 formation were also changed by prolonged treatment with 8Br-cAMP to a monophasic response in which Ins(1,4,5)P3 increased rapidly and remained elevated during All stimulation. In permeabilized glomerulosa cells treated with 8Br-cAMP for 16 h, the conversion of D-myo-inositol 1,3,4-trisphosphate (Ins(1,3,4)P3) to Ins(1,3,4,6)P4 was consistently increased, whereas dephosphorylation of Ins(1,4,5)P3 to D-myo-inositol 1,4-bisphosphate and of D-myo-inositol 1,3,4,5-tetrakisphosphate to Ins(1,3,4)P3, was reduced.

• OSTI ID: 6056498
• Journal Information: Molecular Endocrinology; (USA), Journal Name: Molecular Endocrinology; (USA) Vol. 4:11; ISSN 0888-8809; ISSN MOENE
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADRENAL GLANDS
AMP
ANIMAL CELLS
ANIMALS
BODY
CATTLE
CELL CONSTITUENTS
CELL MEMBRANES
CHEMICAL REACTIONS
DOMESTIC ANIMALS
DOSE-RESPONSE RELATIONSHIPS
ENDOCRINE GLANDS
ESTERS
GLANDS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
LIPIDS
MAMMALS
MEMBRANES
METABOLISM
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PERMEABILITY
PHOSPHOLIPIDS
PHOSPHORYLATION
RUMINANTS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES