Modulation of agonist-induced inositol phosphate metabolism by cyclic adenosine 3',5'-monophosphate in adrenal glomerulosa cells

Journal Article · Thu Nov 01 00:00:00 EST 1990 · Molecular Endocrinology; (USA)

DOI:https://doi.org/10.1210/mend-4-11-1712· OSTI ID:6056498

Baukal, A J; Hunyady, L; Balla, T; Ely, J A; Catt, K J ^[1]

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1. National Institute of Child Health and Human Development, Bethesda, MD (USA)

Activation of the cAMP messenger system was found to cause specific changes in angiotensin-II (All)-induced inositol phosphate production and metabolism in bovine adrenal glomerulosa cells. Pretreatment of (3H)inositol-labeled glomerulosa cells with 8-bromo-cAMP (8Br-cAMP) caused both short and long term changes in the inositol phosphate response to stimulation by All. Exposure to 8Br-cAMP initially caused dose-dependent enhancement (ED50 = 0.7 microM) of the stimulatory action of All (50 nM; 10 min) on the formation of D-myo-inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) and its immediate metabolites. This effect of 8Br-cAMP was also observed in permeabilized (3H)inositol-labeled glomerulosa cells in which degradation of Ins(1,4,5)P3 was inhibited, consistent with increased activity of phospholipase-C. Continued exposure to 8Br-cAMP for 5-16 h caused selective enhancement of the All-induced increases in D-myo-inositol 1,3,4,6-tetrakisphosphate (Ins(1,3,4,6)P4) and myo-inositol 1,4,5,6-tetrakisphosphate. The long term effect of 8Br-cAMP on the 6-phosphorylated InsP4 isomers, but not the initial enhancement of Ins(1,4,5)P3 formation, was inhibited by cycloheximide. The characteristic biphasic kinetics of All-induced Ins(1,4,5)P3 formation were also changed by prolonged treatment with 8Br-cAMP to a monophasic response in which Ins(1,4,5)P3 increased rapidly and remained elevated during All stimulation. In permeabilized glomerulosa cells treated with 8Br-cAMP for 16 h, the conversion of D-myo-inositol 1,3,4-trisphosphate (Ins(1,3,4)P3) to Ins(1,3,4,6)P4 was consistently increased, whereas dephosphorylation of Ins(1,4,5)P3 to D-myo-inositol 1,4-bisphosphate and of D-myo-inositol 1,3,4,5-tetrakisphosphate to Ins(1,3,4)P3, was reduced.

• OSTI ID: 6056498
• Journal Information: Molecular Endocrinology; (USA), Vol. 4:11; ISSN 0888-8809
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CELL MEMBRANES
PERMEABILITY
PHOSPHOLIPIDS
METABOLISM
ADRENAL GLANDS
AMP
ANIMAL CELLS
CATTLE
DOSE-RESPONSE RELATIONSHIPS
PHOSPHORYLATION
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMALS
BODY
CELL CONSTITUENTS
CHEMICAL REACTIONS
DOMESTIC ANIMALS
ENDOCRINE GLANDS
ESTERS
GLANDS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
LIPIDS
MAMMALS
MEMBRANES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
RUMINANTS
VERTEBRATES
550501* - Metabolism- Tracer Techniques