Fiber-optic chemical sensors for competitive binding fluoroimmunoassay

Journal Article · Wed Apr 15 00:00:00 EDT 1987 · Anal. Chem.; (United States)

DOI:https://doi.org/10.1021/ac00135a033· OSTI ID:6803984

Tromberg, B J; Sepaniak, M J; Vo-Dinh, T; Griffin, G D

This paper describes the development of a fiber-optic chemical sensor based on the principle of competitive-binding fluorescence immunoassay. Rabbit immunoglobin G (IgG) is covalently immobilized on the distal sensing tip of a quartz optical fiber. The sensor is exposed to fluorescein isothiocyanate (FITC) labeled and unlabeled anti-rabbit IgG. The 488-nm line of an argon-ion laser provides excitation of sensor-bound analyte. This results in fluorescence emission at the optical fiber's sensing tip. Sensor response is inversely proportional to the amount of unlabeled anti-IgG in the sample. Limits of detection (LOD) vary with incubation time, sample size, and measurement conditions. For 10-/sup +/L samples, typical LOD are 25 fmol of unlabeled antibody in a 20-min incubation period. These results indicate that each fiber-optic fluoroimmunosensor can be constructed to perform a single sensitive, rapid, low-volume immunoassay, in in situ or benchtop applications.

• Research Organization: Univ. of Tennessee, Knoxville
• DOE Contract Number: AC05-84OR21400
• OSTI ID: 6803984
• Journal Information: Anal. Chem.; (United States), Journal Name: Anal. Chem.; (United States) Journal Issue: 8 Vol. 59:8; ISSN ANCHA
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400102* -- Chemical & Spectral Procedures
CHEMICAL ANALYSIS
CHEMICAL LASERS
DATA
EMISSION SPECTROSCOPY
EXPERIMENTAL DATA
FIBERS
FLUORESCENCE SPECTROSCOPY
GLOBULINS
IMMUNOGLOBULINS
INFORMATION
LASERS
NUMERICAL DATA
OPTICAL FIBERS
ORGANIC COMPOUNDS
PROTEINS
QUANTITATIVE CHEMICAL ANALYSIS
SPECTROSCOPY