Construction and Evaluation of a Regenerable Flouroimmunochemical-Based Fiber Optic Biosensor

Technical Report · Fri Dec 14 23:00:00 EST 1990

DOI:https://doi.org/10.2172/6077933· OSTI ID:6077933

Bowyer, James R. ^[1]; Alarie, Jean Pierre ^[1]; Sepaniak, Michael J. ^[1]; Vo-Dinh, Tuan ^[2]; Thompson, Robert Q. ^[3]

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1. Univ. of Tennessee, Knoxville, TN (United States)
2. Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
3. Oberlin College, OH (United States)

A microscale fiber optic biosensor that is capable of in situ regeneration is described and characterized. By combining recently developed fiber optic sensing technology with a capillary column reagent delivery system, it is possible to perform a variety of benchtop affinity assay procedures repetitively and remotely. The configuration of the sensing chamber at the terminus of the fiber is an important design feature. The construction and operation of the sensor is described and the results of evaluations of the sensor using an antibody/antigen system are presented. Affinity assay steps such as the delivery of solid phase affinity reagents, secondary reagents, and rinse solutions are demonstrated. Sampling is accomplished by mild aspiration. Coefficients of variation (CVs) for these steps are all less than 10. The capability of selectivity measuring fluorescently-labeled anti-rabbit-immunoglobulin G (IgG), in the present of a similar protein, utilizing its immunospecific interaction with rabbit-IgG beads, is demonstrated and exhibits a CV of 6.2. A near linear calibration plot is presented over a concentration range of 0.011 mg ml^-1 (approximately the limit of detection) to 0.11mg ml^-1.

• Research Organization: Univ. of Tennessee, Knoxville, TN (United States)
• Sponsoring Organization: USDOE Office of Energy Research (ER); National Science Foundation (NSF)
• DOE Contract Number: FG05-86ER13613
• OSTI ID: 6077933
• Report Number(s): DOE/ER/13613--29; ON: DE91004886; CNN: CHE-8708581
• Country of Publication: United States
• Language: English

Related Subjects

440800* -- Miscellaneous Instrumentation-- (1990-)
47 OTHER INSTRUMENTATION
550200 -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
DATA
EXPERIMENTAL DATA
FIBER OPTICS
FLUORESCENCE
GLOBULINS
IMMUNOGLOBULINS
INFORMATION
IgG
LASERS
LUMINESCENCE
MAMMALS
NUMERICAL DATA
ORGANIC COMPOUNDS
PROTEINS
RABBITS
VERTEBRATES
biosensor
fiber optic
fluoroimmunoassay
laser