Antibody-based biological toxin detection

Conference · Fri Dec 01 04:00:00 EST 1995

OSTI ID:126042

Menking, D E; Goode, M T ^[1]

---

1. Army Edgewood Research, Development and Engineering Center, Aberdeen Proving Ground, MD (United States)

Fiber optic evanescent fluorosensors are under investigation in our laboratory for the study of drug-receptor interactions for detection of threat agents and antibody-antigen interactions for detection of biological toxins. In a direct competition assay, antibodies against Cholera toxin, Staphylococcus Enterotoxin B or ricin were noncovalently immobilized on quartz fibers and probed with fluorescein isothiocyanate (FITC) - labeled toxins. In the indirect competition assay, Cholera toxin or Botulinum toxoid A was immobilized onto the fiber, followed by incubation in an antiserum or partially purified anti-toxin IgG. These were then probed with FITC-anti-IgG antibodies. Unlabeled toxins competed with labeled toxins or anti-toxin IgG in a dose dependent manner and the detection of the toxins was in the nanomolar range.

• OSTI ID: 126042
• Report Number(s): CONF-950402--
• Country of Publication: United States
• Language: English

Related Subjects

44 INSTRUMENTATION
INCLUDING NUCLEAR AND PARTICLE DETECTORS
45 MILITARY TECHNOLOGY, WEAPONRY, AND NATIONAL DEFENSE
55 BIOLOGY AND MEDICINE
BASIC STUDIES
56 BIOLOGY AND MEDICINE
APPLIED STUDIES
ANTIBODIES
BIOLOGICAL WARFARE AGENTS
CHOLERA
DETECTION
FIBER OPTICS
FLUORESCEIN
ISOTHIOCYANATES
LABELLED COMPOUNDS
STAPHYLOCOCCUS
TOXINS