Human alpha-fetoprotein in the epidermal cells of Bowen's disease

Journal Article · Sat Dec 31 23:00:00 EST 1977 · J. Invest. Dermatol.; (United States)

DOI:https://doi.org/10.1111/1523-1747.ep12541327· OSTI ID:5910927

Soltani, K; Yachnin, S; Brickman, F

Human alpha-fetoprotein (HAFP) was demonstrated in clusters of atypical cells of an extensive Bowen's disease involving the pubic area. Frozen sections were studied by indirect immunofluorescence using monospecific rabbit anti-HAFP antiserum or purified rabbit anti-HAFP antibody followed by staining with fluorescein isothiocyanate (FITC)-labeled goat anti-rabbit ..gamma..-globulin and by a direct technique using FITC-labeled rabbit anti-HAFP Fab2 fragments. Sections of fetal liver served as positive controls and sections of adult human skin and liver were used as negative controls. Preabsorption of the anti-HAFP antibodies with purified HAFP blocked the fluorescence. This finding adds Bowen's disease to the list of premalignant disorders which possess HAFP as an oncofetal marker of their malignant potential. HAFP might possibly enhance malignant behavior through interference with cellular immunosurveillance mechanisms.

• Research Organization: Univ. of Chicago, IL
• OSTI ID: 5910927
• Journal Information: J. Invest. Dermatol.; (United States), Journal Name: J. Invest. Dermatol.; (United States) Journal Issue: 4 Vol. 70:4; ISSN JIDEA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ANTIBODIES
AROMATICS
BODY
CARBOHYDRATES
CARBOXYLIC ACIDS
DIGESTIVE SYSTEM
DISEASES
DYES
EMBRYOS
EPIDERMIS
EPITHELIUM
FLUORESCEIN
GLANDS
GLUCOPROTEINS
HYDROXY ACIDS
HYDROXY COMPOUNDS
IMMUNE SERUMS
LABELLING
LIVER
NEOPLASMS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PHENOLS
POLYPHENOLS
PROTEINS
SACCHARIDES
SKIN
TISSUES