Immobilization of antibodies and enzyme-labeled antibodies by radiation polymerization
Immobilization of antibodies and enzyme-labeled antibodies by radiation polymerization at low temperatures was studied. The antibody activity of antibody was not affected by irradiation at an irradiation dose of below 8 MR and low temperatures. Immobilization of peroxidase-labeled anti-rabbit IgG goat IgG, anti-peroxidase, peroxidase, and anti-alpha-fetoprotein was carried out with hydrophilic and hydrophobic monomers. The activity of the immobilized enzyme-labeled antibody membranes varied with the thickness of the membranes and increased with decreasing membrane thickness. The activity of the immobilized antibody particles was varied by particle size. Immobilized anti-alpha-fetoprotein particles and membranes can be used for the assay of alpha-fetoprotein by the antigen-antibody reaction, such as a solid-phase sandwich method with high sensitivity.
- Research Organization:
- Takasaki Radiation Chemistry Research Establishment, Japan Atomic Energy Research Institute, Gunma
- OSTI ID:
- 6385456
- Journal Information:
- Appl. Biochem. Biotechnol.; (United States), Journal Name: Appl. Biochem. Biotechnol.; (United States) Vol. 8:2; ISSN ABIBD
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
400600 -- Radiation Chemistry
560111* -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACRYLATES
ANTIBODIES
ANTIGENS
BIOASSAY
CARBOXYLIC ACID SALTS
CARBOXYLIC ACIDS
CHEMICAL RADIATION EFFECTS
CHEMICAL REACTIONS
CHEMISTRY
ENZYMES
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
IMMOBILIZED ENZYMES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDOREDUCTASES
PEROXIDASES
POLYMERIZATION
PORPHYRINS
PROTEINS
RADIATION CHEMISTRY
RADIATION EFFECTS
SAMPLE PREPARATION