Cultured mouse embryos metabolize benzo(a)pyrene during early gestation: genetic differences detectable by sister chromatid exchange

Journal Article · Sun Jun 01 00:00:00 EDT 1980 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.77.6.3524· OSTI ID:6793270

Galloway, S M ^[1]; Perry, P E; Meneses, J; Nebert, D W; Pedersen, R A

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1. Univ. of California, San Francisco

Mouse embryos explanted at 7-1/2 or 8-1/2 days of gestation were cultured in medium containing benzo(a)pyrene and supplemented with 5-bromodeoxyuridine to allow detection of sister chromatid exchanges. The mirine Ah locus regulates the inducible metabolism of polycyclic hydrocarbons such as benzo(a)pyrene. A high frequency of sister chromatid exchange wass induced by benzo(a)pyrene in embryos from three Ah-responsive inbred strains (BALB/cDub, C3H/AnfCum, and C57BL/6N); there was little or no increase intwo Ah-nonresponsive inbred strains (AKR/J and DBA/2J). Benzo(a)pyrene also induced sister chromatid exchanges in the Ah-responsive recombinant inbred line B6NXAKN-I2 but not in the Ah-nonresponsive recombinant inbred line B6NXAKN-3. Sister chromatid exchange in cultured Ah-responsive mouse embryos was thus shown to be a sensitive assay. These data provide direct evidence that genetically responsive mouse embryos (early postimplantation state) possess the subcellular processes necessaryfor induction of enzymes that metabolize benzo(a)pyrene to its chemically active form(s). Both the Ah regulatory gene product (a cytosolic receptor) and the structural gene product (inducible cytochrome P/sub 1/-450) therefore appear to be functional at an early embryonic age. Furthermore, this metabolic capacity may play an important role in the damage to embryonic cells by polycyclic hydrocarbons.

• OSTI ID: 6793270
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 77:6; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550400 -- Genetics
560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
ANTIMETABOLITES
AROMATICS
AZINES
BENZOPYRENE
BROMOURACILS
BUDR
CELL CULTURES
CHEMICAL ACTIVATION
CHROMOSOMAL ABERRATIONS
CONDENSED AROMATICS
CONTROL
CYTOCHROMES
DRUGS
EMBRYOS
ENZYMES
GENETIC CONTROL
HETEROCYCLIC COMPOUNDS
HYDROCARBONS
HYDROXY COMPOUNDS
MAMMALS
METABOLISM
MICE
MUTATIONS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDOREDUCTASES
PEST CONTROL
PIGMENTS
PREGNANCY
PYRIMIDINES
RIBOSIDES
RODENTS
SISTER CHROMATID EXCHANGES
URACILS
VERTEBRATES