Cytochrome P-450 metabolic activity in embryonic and extraembryonic tissue lineages of mouse embryos

Journal Article · Wed May 01 00:00:00 EDT 1985 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.82.10.3311· OSTI ID:6285016

Pedersen, R A; Meneses, J; Spindle, A; Wu, K; Galloway, S M

Mouse morulae, blastocysts, and embryonic and extraembryonic tissue layers were examined for benzo(a)pyrene metabolism by cytochrome P-450, using the sister chromatid exchange assay. Benzo(a)pyrene exposure in vitro increased sister chromatid exchanges in blastocysts of all genetically responsive mice examined (BALB/cDub, C3H/AnfCum, and outbred Dub: (ICR) strains) but not blastocysts of the nonresponsive AKR/J strain. Benzo(a)pyrene treatment of responsive 7 1/2- and 8 1/2-day (post-implantation-stage) embryos, either intact or as separate tissue layers, increased sister chromatid exchanges in tissues of both embryonic and extraembryonic lineages - i.e., in the embryo proper, in isolated embryonic ectoderm, and in yolk sac, chorion, extraembryonic ectoderm, and in yolk sac, chorion, extraembryonic ectoderm, and extraembryonic endoderm layers. These results indicate that cytochrome P-450 is active in most or all tissues of the early mammalian embyro.

• Research Organization: Univ. of California, San Francisco
• DOE Contract Number: AC03-76SF01012
• OSTI ID: 6285016
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 82:10; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AROMATICS
BENZOPYRENE
BIOCHEMISTRY
BIOLOGICAL EFFECTS
CHEMISTRY
CHROMOSOMAL ABERRATIONS
CONDENSED AROMATICS
CYTOCHROMES
EMBRYOS
GENETIC EFFECTS
HYDROCARBONS
MAMMALS
METABOLISM
MICE
MUTATIONS
ORGANIC COMPOUNDS
PIGMENTS
RODENTS
SISTER CHROMATID EXCHANGES
VERTEBRATES